West China Journal of Stomatology

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Study of increased sensitivity on Tca8113 cell line to cisplatin by nm23-H1 in vitro

ZHI Ke-qian1, REN Wen-hao1, WEN Yu-ming2, MIAO Qun-ai3, LI Hong4   

  1. 1. Dept. of Oral and Maxillofacial Surgery, College of Stomatology, Xi′ an Jiaotong University, Xi′ an 710004, China; 2. Dept. of Oral and Maxillofacial Surgery, West China College of Stomatology, Sichuan University, Chengdu 610041, China; 3. Dept. of Periodontics and Oral Medicine, College of Stomatology, Xi′an Jiaotong University, Xi′an 710004, China; 4. Dept. of Stomatology, Lintong Sanatorium of Chinese People′s Liberation Army, Lanzhou Military District, Lintong 710600, China
  • Received:2008-10-25 Revised:2008-10-25 Online:2008-10-20 Published:2008-10-20
  • Contact: ZHI Ke-qian，Tel：029-81015479

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Abstract:

Objective To study the mechanism of sensitivity variation to cisplatin caused by nm23-H1. Methods The samles was divided into two groups: Tca8113 group and Tca8113/nm23-H1 group. Using MTT and flow cytometer, the changes of cell mortality rate, apoptosis and mitochondrial membrane potential were detected. By VG PQ Excell, the changes of the intracellular platinum were detected. Results In vitro the cell mortality rate and apoptosis were increased in Tca8113/nm23-H1 group, comparing with Tca8113 group. Mitochondrial membrane potential was decreased in Tca8113/nm23 -H1 group. The intracellular platinum was increased significantly in Tca8113/nm23 -H1 group. This effect could be inhibited by oubain which was an inhibitor of Na+/K+-ATP. Conclusion nm23-H1 can increase the sensitivity of cisplatin on Tca8113 cell line. The mitochondrial membrane potential was decreased by nm23-H1 so that intracellular platinum was increased and finally increased the apoptosis or necrosis.

Key words: cisplatin, chemotherapy, tonguecancer

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