Abstract:

Objective To investigate whether the exosomes derived from Tca8113 could induce production of tumor-specific  T cells when pulsed onto dendritic cells. Methods  Tca8113 cell was cultured with RPIMI640. isolated and purified the tumor-derived exosomes from the culture supernatants by ultrafiltration with Millipore centrifual filter devices; frozen and thawed Tca8113 cells to get frozen tumor antigens(FTA). The exosomes and FT A was pulsed onto DC generated from normal human peripheral blood mononuclear cell (PBMC) in vitro. The DC pulsed with FAP or exosomes cocultured with the peripheral blood lymphocytes to transform T cell into specific CTL To observe the killing and  wounding activity of CTL, the CTL and Tca8113 cells were mixed at a ratio of 20 to 1, SPCA-l cells and 95-D cells was evaluated as control group. Results The CTL induced by DC pulsed with FAP or exosomes had significant activity killing Tca8113 CP

Key words: Tca8113cell, cytotoxicTlymphocyte, dendriticcell