Abstract:

Objective To investigate the interference and the mechanisms of Bcl-2 and HER-2 genes antisense oligodeoxynucleotides combined transfection in the human tongue carcinoma Tca8113 cell lines. Methods There were 6 groups in our study, normal control group, Bcl-2 sense experimental group, HER -2 sense experimental group, Bcl-2 antisense experimental group, HER-2 antisense experimental group, Bcl-2 and HER-2 genes antisense oligodωxynucleotides combined transfection experimental group. ln the different times after liposome-mediated transfection, the cell apoptosis, Bcl-2 and HER-2 exressing level were observed by RT-PCR and electronic microscope. ResuIts According to the results of combined transfection experimental group, the apoptosis body and apoptosis cells were observed. The expression of genes were decreased statistically in both Bcl-2 and HER-2, respectively. Bcl-2 and HER-2 combined ASODN was superior to single ASODN in the intervention of tongue carcinoma. Conclusion Bcl-2 and HER-2 ASODN can effectively interfere the expression of HER-2 and Bcl-2 genes. The expression of HER-2 and Bcl-2 can be reduced, and the apoptosis of cells can be enhanced.

Key words: Tca8113celllines, HER-2andBcl-2genes, antisenseoligodeoxynucleotides, combinedtransfection