West China Journal of Stomatology
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WANG Jing, WU Jun-zheng, GUO Fu-ping, ZHU Xiu-li, WEN De-sheng
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Abstract:
Objective To construct the plasmid containing short hairpin RNA(shRNA) of TGF-β1 expression vector. Methods Short chain oligonucleotide was designed according to the TGF-β1 mRNA sequence provided by Genebank, then DNA segment was gained through annealing after chemosynthesis, and then was cloned to pWH1 vector. The recombinant TGF-β1 shRNA expression vector was evaluated by using enzyme cutting. At last, the constructed TGF-β1 expression vector was transfected into salivary gland mucoepidermoid carcinoma(Ms) cells by Lipofectomine TM 2000, and its effect on TGF-β1 expression was observed by RT-PCR and immunohistochemistry. Results Successful construction was identified by enzyme cutting and the constructed plasmid was called pWH1-TGF-β1. The shRNA and it inhibited the TGF-β1 mRNA and protein expression effectively. Conclusion The constructed TGF-β1 shRNA expression vector can block the TGF-β1 expression in salivary gland mucoepidermoid carcinoma cells.
Key words: shorthairpinRNA, ukaryoticexpressionvector, ransforminggrowthfactor-β1
WANG Jing, WU Jun-zheng, GUO Fu-ping, ZHU Xiu-li, WEN De-sheng. Construction of Eukaryotic Expression Vector of Short Hairpin RNA for Transforming Growth Factor-β1[J]. West China Journal of Stomatology.
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https://www.hxkqyxzz.net/EN/Y2006/V24/I02/113