Abstract:

bjective To investigate the role of c-Jun and c-Fos as transcriptional factors in regulation of dentin sialophosphoprotein（DSPP） gene by a promoter-luciferase reporter gene construct in odontoblast cell line MDPC-23. Methods Endogenous c-Jun or c-Fos protein was determined by immunocytochemistry. The role of c-Jun or c-Fos in transcription of DSPP was investigated in co-transfection experiments using promoter-luciferase reporter gene construct containing the sequence between -791 bp and +54 bp of mouse DSPP gene. Results c-Jun and c-Fos was expressed by MDPC-23 cells, and located in the nucleus of MDPC-23 cells. Overexpression of c-Jun or c-Fos significantly inhibited luciferase activity of DSPP promoter. Conclusion These findings suggest c-Jun and c-Fos downregulated the transcription of DSPP gene as a transcriptional factor in odontoblast.

Key words: dentinsialophosphoprotein, c-Jun, c-Fos, genetranscription