Abstract:

Objective  To investigate the effects of substance P on cultured rat osteoclasts. Methods  Neurokinin-1（NK1）receptor expression in osteoclasts was examined by immunohitochemical method, and changes of bone resorption activity caused by substance P and NK1 receptor antagonists were detected by pit formation assay. Results  Immunoreactivity for NK1 receptor was distributed in the cytoplasm of osteoclasts. The average of pit formation areas significantly increased with addition of  substance P （10-7-10-4 mol/L） （P<0.05）, but the number of pitformations did not change（P>0.05）. NK1 receptor antagonists inhibited the enhancement of the bone resorption by substance P addition. Conclusion  The findings suggested  that substance P may stimulate osteoclasts and result in bone resorption by the mediation of NK1 receptor.

Key words: substanceP, Neurokinin-1receptor, Neurokinin-1receptorantagonist, osteoclasts