Abstract:

Objective　To investigate the relationship of vascular endothelial growth factor c(VEGF-C) and induced nitride ox- ide synthesizase(iNOS) expression in lymph node micrometastasis of oral squamous cell carcinoma.Methods　Samples were ob- tained from 47 cases of oral squamous cell carcinoma and 15 caseswith normal oral mucosa, VEGF-C and iNOSmRNA expression were detected by RT-PCR method. Lymph node micrometastasis of 10 normal lymph nodes and 355 lymph nodes from 47 cases of oral squamous cell carcinoma was detected with immunohistochemical reaction in cytokeratin antibody.Results　The percentages in tumorswith higher expressionwere 57·4% forVEGF-C, 68·1% for iNOS(P<0·05). Theywere significantly higherthan that of normal groups. Significant positive relationshipwas found between VEGF-C and iNOS(P<0·01). The positive rate of cytoker- atin(CK) was 48·9%. Significant positive relationship was found between VEGF-C and CK, iNOS and CK(P<0·01). The ex- pression rates of CK in positive group of VEGF-C and iNOS were 63·0%,65·6% respectively,and were significant higher than negative groups.Conclusion　Expression of VEGF-C and iNOS in lymph node micrometastasis of oral squamous cell carcinoma is significant related.

Key words: micrometastasis, vascular endothelial growth factor C, induced nitride oxide synthesizase