West China Journal of Stomatology

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Investigation of the Expression of Calcitonin Receptor mRNA in Human Osteoclasts on Deciduous Teeth

CHENMing1, LIANGXing2, BAOXiang-jun2, WANGHang2, SUNHui-qiang2,LUShao-heng2   

  1. 1.Key. Laboratory ofOral Biomedical En- gineeringMinistry ofEducation, Sichuan University, Chengdu610041, China;2.Dept. ofProsthetics, West China College of Stomatology, Sichuan University, Chengdu610041, China
  • Received:2004-06-25 Revised:2004-06-25 Online:2004-06-20 Published:2004-06-20

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Abstract:

Objective　To investigate the expression of calcitonin receptor mRNA in the osteoclasts of the resorbing deciduous teeth.Methods　After fixing the collected deciduous teeth, toluidine blue was performed and tartrate-resistant acid phosphatase (TRAP) staining was used to identify the osteoclasts on the resorbing surface of human deciduous teeth and in situ hybridization of calcitonin receptor mRNA to show its existence.Results　There were a number of TRAP positive osteoclasts on the root surface which showed the expression of calcitonin receptor mRNA.Conclusion　On the resorbing surface of human deciduous teeth there are osteoclasts that express calcitonin receptormRNA, so it is feasible to use this kind of osteoclasttotestthe effect of external fac- tors on the expression of CTR mRNA.

Key words: deciduous teeth, osteoclasts, in situ hybridization

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