Abstract:

Objective　To establish a new culture systerm for mouse tooth germs in chick embryo.Methods　The mandibular first molar germ fragments of 15 embryonic days′Kunming mouse embryo were implanted into the lateral mesenchyme of 4~5 days′chick embryo wing buds in ove. Eggs were reincubated and implanted tissues were examined by histochemistry.Results　 The cultured tooth germ development continued from cap stage to latest bell stage. The ameloblast and the odontoblast all differen- tiated maturely and secreted matrix.Conclusion　4~5 days′wing buds chick embryo could serve as developing the mouse tooth germs and demonstrate well physiological process of differentiation and morphogenesis.

Key words: mouse, tooth germ, chick embryo, culturein vivo