Abstract:

Objective　This study is to make sure if the constructed pcDNA3 carrying encoding gene of sIL-1R can be expressed in mammalian cellsin vitro.Methods　COS-1 cells and CHO cells were respectively transfected with recombinant plasmid pcDNA3/sIL-1R by liposome. The protein expression products were detected by ELISA.Results　The results indicated that the protein expression products could be detected in the cell plasma and the cell culture supernatant. The expression level in experimental groups was much higher than that in control groups(P<0·05).Conclusion　The constructed pcDNA3/sIL-1R can express interest protein in mammalian cells and this establishes the basis for future investigation on gene therapy of periodonititis and other inflammatory diseases.

Key words: human soluble interleukin-1 receptor, mammalian cells, in vivotransfer, gene therapy