Abstract:

Objective: The aim of this study was to investigate the expression of Smadl gene in human dental papillae cells (HDPC) and the transduction process and, to explore the molecular mechanism of odontoblast differentiation during tooth development process at a signaling transduction level. Methods: Immunohistochemical staining of HDPC derived from primary culture stimulated by transforming growth factor B1(TGF-B1) and recombinant human bone morphogenetic protein (rhBMP2) were used in this investigation. Results: The expression of protein of Smadl gene was found in HDPC stimulated by TGF-ji and rhBMP2 , but no significant difference was found between these experimental groups and the control group. Smad2 was found nuclear translocation accumulation form cytoplasm, when HDPC were treated by rhBMP2 .Conclusion: The expression and translocation accumulation of the protein of Smadl gene in HDPC were detected at the first time. The signaling transduction of rhBMP2 in dental papilla cells could be mediated by Smadl translocation and accumulation in nucleus.

Key words: human dental papillae cell, smadl, transforming growth factor B1, recombinant human bone morphogenetic protein 2