Abstract:

Objective:The purpose of this studywas to investigate the relationship between the results of flowcytometry analyses of differ- ent clinical stage, location, pathologic grade and cell origin of oral and maxillofacial non-Hodgkincs lymphoma (NHL), and the diagnostic value of flowcytometry analysis in lymphoma.Method:This study analyzed 50 oral and maxillofacial NHL cases and 10 reactive lymph nodes (formalin fixed and paraffin embedded) by flowcytometry (FCM).Results:Reactive lymph nodes were all diploid. The diploid rate of NHLwas 54%, and aneuploidy rate was 46%. There was statistically significant difference between reactive lymph nodes and NHLin the DNA ploidy status and cell cycle data (SPF, CV, S+G2/M, DI). The S phase fraction (SPF) and S+G2/M had close relationshipwith the grade of NHL. SPF value and DNA ploidy status had no obvious relationship with the prognosis.Conclusion:The results suggested that the FCMhad diagnostic value inNHL, especiallywhen the morphologi- cal diagnosis was difficult. Although the cell cycle data had no prognostic value, SPF and SPF+G2/M can show the proliferative status of NHL, which can help clinical doctor select therapeutic method.

Key words: flowcytometry, non-Hodgkin&prime, slymphoma, reactivelymphnodes