Abstract:

Objective:The purpose of this study was to investigate the effects of epidermal growth factors (EGFs) with different concen- tration on the OD, DNA, protein, and PGE2of A/J mouse embryonic palatal shelves cells (A/J MEPC) isolated from embryonic palatal shelves.Methods:The mouse embryonic palatal shelves cellswere grown in different 39 pores (or bottles) with 9 gradient concentrations of EGF (01005, 01010, 01050,01100, 01500, 11000, 51000, 101000, 501000 ng/ml), and four pores were prepared for the same concentration, then the OD, DNA, protein and PGE2of A/JMEPCwere measured after1 day, 3 days and 5 days.Results:EGFs stimulated DNA and PGE2synthesis of A/JMEPC, and augmented proliferation index (PIX). Their effects were very obvious in promoting the proliferation of A/JMEPC, when the concentrationwas 101000 ng/ml.Conclusion:EGFmay be important in regulating proliferation and metabolism of embryonic palatal shelves cells.

Key words: epidermal growth factors, cell culture, proliferation and metabolism, embryonic palatal shelves