Abstract:

The aim of this study was to investigate suppression effects of the transfection of human tumor necrosis factor- α (hTNF-α) gene on tongue carcinoma cells.Methods : The shuttle plasmid containing hTNF-α gene was extracted and purified, then it was transferred into Tca8113 tongue carcinoma cells with cationic liposome DOSPER. The control group was only given equivalent liposomes , except the plasmid. After culturing for 24 , 48 , 72 and 96 hours , the expression of hTNF- αgene in Tca8113 cells was analyzed by ELISA and , the survival rate of transferred cells was assayed byMTT enzymatic labeling technique.Results : The transferred Tca8113 cells displayed significantly overexpression of hTNF-α ( P < 0. 05) . The survival rate of the transferred Tca8113 cells was decreased significantly ( P < 0. 05) . Conclusion : Transfection of hTNF-αgene in vitro mediated by cationic li2 posomes can induce the overexpression of hTNF-αand inhibit the growth of tongue carcinoma cells.

Key words: human tumor necrosis factor-α, tongue carcinoma, gene transfection , cationic liposome