Abstract:

Objective:The aim of this study is to investigate temporal and spatial change patterns of osteocalcin in serum of young grow- ing SD rats, in conditions of different functional treatment time spans.Methods:Totally, 72 SD rats are randomly divided into 3 groups, including the control group, the 24-hourwearing group (wearing functional appliance 24 hours a day), and the 12-hour wearing group (wearing appliance for 12 hours in daylight). One week after the experiment, rats were sacrificed and osteocalcin level in serumwasmeasured by using radioimmunoassy. Date was processed using macroscopic and microscopic analysis.Results: Serum osteocalcin had circadian rhythm and the peakwas about12:00 in a day. Afterwearing the functional appliance, serum os- teocalcin level increased with treatment time, and serum osteocalcin level in the 24-hour wearing group rose more obviously than that in the 12-hourwearing group.Conclusion: Forthe functional appliance, better results can be achieved in the 24-hourwearing group.

Key words: functional treatment, osteocalcin, circadian rhythm