Acquisition of submandibular gland in mouse embryo and establishment of an in vitro organ culture model

doi:10.7518/hxkq.2020.02.020

West China Journal of Stomatology ›› 2020, Vol. 38 ›› Issue (2): 228-232.doi: 10.7518/hxkq.2020.02.020

Acquisition of submandibular gland in mouse embryo and establishment of an in vitro organ culture model

Liu Zhikai¹, Qiao Xianghe¹, Gou Liming², Li Chunjie¹()

^1. State Key Laboratory of Oral Diseases & National Clinical Research Center for Oral Diseases & Dept. of Head and Neck Oncology, West China Hospital of Stomatology, Sichuan University, Chengdu 610041, China
^2. Chongqing Key Laboratory of Oral Diseases and Biomedical Sciences, Stomatological Hospital of Chongqing Medical University, Chongqing 401147, China

Received:2019-07-04 Revised:2020-01-20 Online:2020-04-01 Published:2020-04-15
Contact: Chunjie Li E-mail:lichunjie@scu.edu.cn
Supported by:
The National Natural Science Foundation of China(81870782);Innovation Spark Project of Sichuan University(2018SCUH0055)

Abstract

Abstract:

Branching morphology is important to ensure that the organ can obtain the efficient functional morphology in a limited volume. The submandibular gland is a crucial model for studying the morphological processes of organ branches. Harvesting the submandibular gland from mouse embryo is also an essential research technique. In this paper, a modified method for obtaining the submandibular glands of mouse embryo was introduced, and the whole process of obtaining and establishing in vitro organ culture was briefly introduced to accurately simulate branch morphogenesis for vivo development and related research.

Key words: mice, embryo, submandibular gland, in vitro organ culture, branching morphogenesis

CLC Number:

Q813.1⁺3

Liu Zhikai, Qiao Xianghe, Gou Liming, Li Chunjie. Acquisition of submandibular gland in mouse embryo and establishment of an in vitro organ culture model[J]. West China Journal of Stomatology, 2020, 38(2): 228-232.

Figures/Tables 4

References 11

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Acquisition of submandibular gland in mouse embryo and establishment of an in vitro organ culture model

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