Abstract:

Objective To study the influence of the quorum sensing inhibitor brominated furanone on Porphyromonas gingivalis（P.gingivalis） biofilm formation. Methods Doubling dilution method was used to determine the minimal inhibition concentration（MIC） of brominated furanone on P.gingivalis. Absolute ethyl alcohol added in P.gingivalis bacterial suspension was used as the negative control, while P.gingivalis bacterial suspension as blank control. The influences of 1/4MIC, 1/2MIC, MIC, 2MIC of brominated furanone on P.gingivalis biofilm formation were studied by the optical density determination and scanning electron microscope（SEM）. Results Four groups of brominated furanone with different concentrations were shown to inhibit P.gingivalis biofilm formation. With the increased concentration of brominated furanone, optical density of P.gingivalis suspension decreased. The biofilm structures of 1/4MIC group, 1/2MIC group and MIC group were loose. Only scattered P.gingivalis cells but no biofilm structure was seen in 2MIC group. Conclusion Brominated furanone could inhibit P.gingivalis biofilm formation without the influence on bacterial growth. The future application of this chemical compound may provide a new possibility for the antimicrobial treatment of periodontal disease.

Key words: Porphyromonas gingivalis, biofilm, brominated furanone