Abstract:

Objective To probe into the function of stem cell antigen-1（Sca-1） in cell proliferation and differentiation. Methods Sca-1+ and Sca-1- muscle derived cells were separated from C57BL/6 mice by fluorescence-activated cell sorting and then cultured in vitro. After 5 days cells proliferative curve were drawn according CCK-8 experimental results. Western blot also were done to detect Sca-1, MyoD and Myogenin expression in cultured Sca-1+ and Sca-1- muscle derived cells. Results The difference of the proliferative curve of Sca-1+ and Sca-1- muscle derived cells cultured 3 days in vitro was not apparent, but Sca-1- muscle derived cells had a accelerated division rate in the follow days compared to the Sca-1+ muscle derived cells. Sca-1 expression in both cells was not obvious. MyoD and Myogenin expression were stronger in Sca-1+ than Sca-1- muscle derived cells. Conclusion Sca-1 expression in muscle derived cells takes a period of time that related to the beginning and ending of the cell cycle.

Key words: stem cell antigen-1, muscle stem cell, muscle regulatory factor, cell cycle