Abstract:

Objective To evaluate the effects of Drynaria fortunei naringin on the total protein content and ultra- structure of human periodontal ligament cells（hPDLCs）. Methods Through enzyme digestion combined tissueculture method, primarily culture and identify the human periodontal ligament cells. Coomassie brilliant blue staining was used to detect the total protein content of hPDLCs with the effects of difference concentration of Drynaria fortunei naringin at difference times. Transmission electron microscope was used to study the ultra-structure of hPDLCs with the effects of Drynaria fortunei naringin. Results In vitro, the addition of Drynaria fortunei naringin at dose of 100, 10, 1, 0.1 mg·L-1 in cultures resulted in an increase of total protein content at the 3rd, 5th, 7th day, but the maximum response was obtained with 1 mg·L-1 Drynaria fortunei naringin. There were more rough endoplasmic reticulums, mitochodrias and ribosomes in the experimental group than in the control. Conclusion Drynaria fortunei naringin may stimulate the protein synthesis and metabolism of hPDLCs.

Key words: Drynaria fortunei, naringin, periodontal ligament cells