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Table of Content
20 April 2005, Volume 23 Issue 02
  • The Distribution offimA Genotype ofPorphyromonas gingivalisin Chronic Periodontitis Patients
    GUO Yong-hua1, WU Ya-fei2, LIUTian-jia2, XIAOXiao-rong1, ZHOUBin3, ZHOUXue-ping3
    West China Journal of Stomatology. 2005, 23(02):  99-102. 
    Abstract ( 877 )   HTML ( 0 )   PDF(pc) (159KB) ( 435 )   Save
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    Objective To investigate the distribution offimA genotype ofP. gingivalisin chronic periodontitis patients. Methods Subgingival plaque sampleswere collected from 101 chronic periodontitis patients.P. gingivaliswas detected by both culture method andP. gingivalis16S rRNAPCR.fimAtype-specific primerwere designed,and the distribution offimAgenotype ofP. gingivalisin periodontitis patients were detected by PCR.Results The detective ratio ofP. gingivaliswas 88.1%. A- mong them, a singlefimAgenotype was detected in most subgingival plaque samples (65.1%), and the distribution of fivefimA genotypes amongP. gingivalispositive patients was as follows: typeⅠ, 24.7%; typeⅡ, 43.8%; typeⅢ, 15.7%; type Ⅳ, 40.4%; typeⅤ, 3.4%; respectively.Conclusion P. gingivaliswith variousfimAgenotypeswere present in subgingival plaque samples from chronic periodontitis patients, andP. gingivaliswith typeⅡfimA andⅣfimA were more predominant in chronic periodontitis patients, and they may be associated with the development of periodontitis.

    Influence of Dexamethasone on Fusion of Embryonic Palatal Medial Edge Expithelium in Mouse Palatal Shelvesin vitro
    HUANGLei1, SHI Bing2, SUNJin-hu3, WANG Yan2
    West China Journal of Stomatology. 2005, 23(02):  103-105. 
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    Objective To study the effect of dexamethasone on the differentiation and proliferation of type A mouse palatal medial edge epithelial cells when there is type A mouse embryonic palatal mesenchymal cells.Methods The mouse palatal shelves were harvested from a female mouse of gestation day 14 by microsurgical dissection and culturedin vitro. The differentia- tion was investigated through microscope and transmission electron microscope under condition of the palatal shelves fusion.Re- sults Dexamethasone promoted the palatal medial edge epithelium differentiated into squamause epithelium and affected normal development and obstructed the fusion of mouse palatal shelves.Conclusion The results of histological observation indicate that dexamethasone promotes the proliferation of palatal meseuchymal cells and inhibits the normal differentiation of palatal medial edge epithelial cells, which results in cleft palate.

    The Study of Cell Biocompatibility of New Pattern Biphasic Calcium Phosphate Nanocompositein vitro
    WANG Tao, TIANWei-dong, LIULei, CHENGXi-zhe, LIAO Yun-mao, LISheng-wei.
    West China Journal of Stomatology. 2005, 23(02):  106-109. 
    Abstract ( 913 )   HTML ( 0 )   PDF(pc) (190KB) ( 518 )   Save
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    Objective To study the cell biocompatibility of porous biphasic calcium phosphate nanocompositein vitro.Meth- ods Bone marrowmesenchymal cell(BMSCs) obtained from SD rat bone marrow werein vitroinduced and proliferated. After theirosteoblast phenotypes were verified, BMSCs were seeded onto prepared porous biphasic calcium phosphate nanocomposite (Experiment group) and common porous hydroxyapatite (Control group). The cell adhesion was evaluated by scanning electron microscope. Synthesis of alkaline phosphatase enzyme (ALP) and osteocalcin were detected and cell cycle was detected by flow cytometry.Results BMSCs could fully attach to and extend on the material in experiment and control group, Moreover, experi- ment groupwere superiorto control group in adhesion, proliferative abilities and osteogenic activity.Conclusion BMSCs can dif- ferentiate to osteoblast phenotype; the porous biphasic calcium phosphate nanocomposite as bone tissue engineering scaffold has good cell biocompatibility.

    The Changes of Cytoskeleton F-actin in Rat Bone Marrow Mesenchymal Stem Cells and Calvarial Osteoblasts under Me- chanical Strain
    QIMeng-chun, HUJing, ZOUShu-juan, HANLi-chi, LUO En
    West China Journal of Stomatology. 2005, 23(02):  110-112. 
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    Objective To explore the response of rat bone marrowmesenchymal stem cells (MSCs) and calvarial osteoblasts to mechanical strain and the consequent changes of cytoskeleton F-actin.Methods Bone marrow MSCs and calvarial osteoblasts were isolated from SD rats and culturedin vitro. Mechanical stretchwas performed on passage 3 cells at 2 000μεfor 0, 2, 6 and 12 hours using four-point bending system. The response of cells and the distribution of F-actin were observed using fluorescent staining under laser scanning confocal microscope and the morphological parameters were quantified using image analysis software Laserpix.Results Under mechanical stretch, the fluorescent staining decreased obviously at bothMSCs and osteoblasts, and F- actin filaments were rearranged and became tenuous, thinner, and abnormally distributed. The outline of nucleus became unclear and apoptotic changes were observed at some of both cells. Cellular size decreased more significantly inMSCs than in osteoblasts. Quantity analysis showed that total area of cells, total fluorescent density and green fluorescent density (F-actin) were all signifi- cantly decreased in MSCs(P<0·05 orP<0·01), and total fluorescent density, green fluorescent density and red fluorescent density (nuclei) did also in osteoblasts(P<0·05 orP<0·01).Conclusion Mechanical stretch caused extensive response on both MSCs and osteoblasts which led to the rearrangement of F-actin filament and apoptosis in some of these cells. MSCs were more sensitive to mechanical strain than osteoblasts.

    The Effect of Matrix Metalloproteinase-1 on Root Surface Dentin Matrix: a Scanning Electron Microscope Observation
    HE Chang-li1, WANG Duo1, LIUZhen-hua1, JINJie2, Gong Yan-hong3
    West China Journal of Stomatology. 2005, 23(02):  113-115. 
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    Objective To observe the effect of matrix metalloproteinase-1(MMP-1) from human host on degradation of dentin organic matrix of root dentin.Methods The freshly extracted caries-free impacted teethwere selected. Teethwere cuttransverse- ly under the enamel-cementum junction into dentin sections with a thickness of about 5 mm. Then all sections with removal of ce- mentum, pulp and predentin were randomly divided into four groups. In the first group, dentin sections were demineralized with acid solution for 21 days, and then incubated withMMP-1 solution for 7 days; the second group were only treated with acid solu- tion for 21 days; the third group were only attacked byMMP-1 solution for 7 days; and the fourth group were untreated as a con- trol. Then all sectionswere dehydrated in ascending strength of alcohol, critically dried, coated with platinum, and then observed under scanning electron microscope(SEM).Results The dentin sections of root surface attacked by acid andMMP-1 showed that demineralization of dentin mineral and degradation of dentin matrix fibrae synchronously happened. The dentin matrix fibrae wasn′t degradated in the groups treatedwith acid orMMP-1.Conclusion The proteinases from human hostmay play an important role in the development of root surface caries. MMP-1 may distinctly degradate the organic matrix of demineralized dentin.

    Study on Lactate Dehydrogenase Activity ofStreptococcus mutansIsolates Derived from Caries-active and Caries-free In- dividuals
    West China Journal of Stomatology. 2005, 23(02):  116-118. 
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    Objective To preliminarily investigate the relationship between the Lactate dehydrogenase (LDH) activity ofStrep- tococcus mutans(S.mutans)and dental caries initiation.Methods 100S. mutansstrains derived from caries-active and caries- free individuals were cultured in BHI medium supplemented with glucose. Cells were extracted and ruptured, and the extracted liquid proteinwas quantifiedwith Coomassie brilliant blue G250 stainingmethods. LDHactivitywas assayed usingthe pyruvate-de- pendent oxidation of NADH-with and without FDP.Results LDH activity of the two groups strains had no difference (P> 0·05), but the distribution of differ class enzyme activity strains in the two groups was different (P<0·05).Conclusion LDH activity ofS.mutansis correlated to the initiation of dental caries to some extent. The measurement methods in this study can be applied in preliminary quantitation of LDH activity and the screening ofS.mutansstrains.

    Clinical Analysis of 1 423 Cases of Root Canal Therapy
    YINShi-hai, YANGJin-bo, SUQin, HUANGDing-ming, CHEN Lei
    West China Journal of Stomatology. 2005, 23(02):  119-121. 
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    Objective To evaluate the clinical quality of root canal therapy (RCT) in West China Dental Hospital of Sichuan University.Methods 1 423 RCTteeth were finished fromMar. 2001 to Feb. 2002 in West China Dental Hospital. Root canal filling quality and treatment period of these teethwere evaluated. 695 teeth of the totalwere revisited 2 years later and 2-year suc- cess rate were evaluated.Results The ratios of adequate filling, underfilling, and overfilling were 79·97%, 14·62% and 5·41%, receptively. Full canal RCTratio of molarwas 89·44%. Average RCTtreatment period was 2·8 weeks. 2-year success rate of RCTwas 94·39%.Conclusion Clinical RCT level of West China Dental Hospital was satisfactory from 2001 to 2002.

    Effect of Frankel Function Regulator on the Condylar and Mandibular Positions of Patients with ClassⅡMalocclusion 
    WANG Yan-min1, ZHOULi1, YIXin-zhu2, CHEN Yang-xi1
    West China Journal of Stomatology. 2005, 23(02):  122-125. 
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    Objective To determine the positional changes of condyle and mandible in children treated successfully with Frankel function regulator.Methods 30 Angle′s classⅡpatients including 15 boys and 15 girls treated with Frankel function regulator were selected. Superimposition of the pretreatment and posttreatment lateral cephalograms of all the patients was done on the stable bone structure of the anterior cranial base and mandible. Cartesian coordinate systemwas used to measure the positional changes of condyle and mandible over time by computer.Results There was significant inferior displacement of condyle, gonion and pogonion after treatment with reference to stable bone structure of anterior cranial base. Anterior displacement of pogonion in boys was also significant. There was significantly superior and posterior displacement of condyle and posterior displacement of go- nion after treatment with reference to stable bone structure of mandible.Conclusion Frankel function regulator can stimulate condylar growth and do favor to mandibular remolding.
    A Study on Initial Colonization ofMutans streptococcion Teeth in Infants and Toddlers
    WANGJun-xiang, GELi-hong, ZHENG Shu-guo
    West China Journal of Stomatology. 2005, 23(02):  126-127. 
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    Objective To investigate the initial colonization ofMutans streptococci (MS) on teeth of children.Methods  Dental plaque was collected from 123 children aged from 7 to 42 months.MSwas isolated by culturing on selective medium and i- dentified by biochemical tests.Results MSwas detected in 51 of 123 children. The rate of colonization was 41·5%, and the mean age of colonizationwas 29 month. The incidence ofMSinfection increasedwith age and tooth eruption, and reached peak at 25~31 months, whichwas statistically significant. There was a correlation between infection ofMSand dental caries.Conclusion  The results indicate that the susceptive period ofMSinfection is from 25 to 31 months when the second primary molars erupt. MSinfection is associated with dental caries.

    The Changes of Bacteria Group on Oral Mucosa after Radiotherapy of Postoperative Patients of Oral Carcinoma
    LIU Kun1, GAONing2, WANG Yi-chuan3, WANG Chang-mei1, WANGXiao-yi1, GAO Qing-hong1, XUANMing1, WENYu-ming1.
    West China Journal of Stomatology. 2005, 23(02):  128-129. 
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    Objective To investigate the microbial contents presented on the surface of mucosa in the oral cavity of patients who accepted radiotherapy, and to provide the evidences of controlling post-radiotherapeutic infections.Methods 32 patients (19 males and 13 females) aged from 37~72 received radiotherapy after oral squamous cell carcinomas operationwere selected. Sam- ples of salivawere obtained fromthe radiated center and opposite mucosa before and after radiotherapy. The detective amount, de- tective ratio and constituent ratio were analysed by cultivation and identification.Results Streptococci, Candida albicansand Pseudomonas aeruginosasignificantly increased on both sides of the oral mucosa whileNeisseriaandActinobacillusdecreased on radiated region after the radiotherapy.Conclusion Radiotherapy has great effects on oral bacteria and pathogenic organism may play a role in post-radiotherapy infections. It is necessary to do bacteria culture and choose sensitive antibiotics regularly for post- radiotherapeutic patients.
    The Effects of Desensitizing Bonding System for Prevention of Vital Abutment Hypersensitivity
    ZHANGHuai-qin, CHAO Yong-lie
    West China Journal of Stomatology. 2005, 23(02):  130-132. 
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    Objective To evaluate the effects of GLUMA desensitizing bonding system in desensitizing hypersensitivity of vital abutment.Methods 69 central incisors with vital pulp from 69 patients were randomly divided into A, B and C group. After tooth preparation, group A coated with primer after etching, group B coated with primer after etching cervical dentin only, and group C was control group without special treatment. The cold sensitivity of abutments was tested after coating with primer, after cementation of crown and three months later. Two fresh extracted caries and restoration-free middle incisorwere prepared in routine way. The surface of specimen 1 was etched with conditioner and the control specimen 2 was prepared without any special surface treatment. Scanning electron microscope(SEM) observation was used to examine the details on dentin surface.Results In all three times of examination, the sensitivities of two experimental groups had been significantly reduced compared with the control group. Immediately after coating, group B were more sensitive than group A (P<0.05). After cementation of crown and three months later, there was no statistically significant between group A and group B. SEMphotomicrograph showed that the smear lay- er was removed from etched dentine surface with open dentinal tubules.Conclusion Coating with GLUMA desensitizing bonding system could prevent hypersensitivity of vital abutment.

    Effects of Varying the Opaque Thickness and the Type of Metal Ceramic Alloy on Color
    PEI Yan-ping1, CHENJi-hua2, CHANGQing2, ZHAO Gui-wen2,XIE Bai-mei2
    West China Journal of Stomatology. 2005, 23(02):  133-135. 
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    Objective To compare the color differences of different metal bases and different opaque thickness, and evaluate the best thickness of opaque on differentmetal bases.Methods Preciousmetal, gold sediment and Ni-Cr plateswere prepared as bases, then opaque samples of 0.05, 0.1, 0.2, 0.3, 0.4 and 0.5 mm thickness were fabricated on such plates. Minolta chro- matics meter CR-321 was used to examine the color properties of these samples, andΔEwas calculated to evaluate the color differ- ence of the specimens.Results For the restoration based on Ni-Cr and precious alloys, the color of restorations was not affected by the color of metal bases as the thickness of opaque reached 0.3 mm, and the chromatic valueΔE<1.5 NBS. For the restora- tion based on gold sediment, the color of restoration was not affected by the color of metal as the thickness of opaque reached 0·1 mm, and the chromatic valueΔE<1.5 NBS.Conclusion Different opaque thicknesswas necessaryto obtain ideal color ap- pearance in clinic. As the opaque thickness increased, the color of restoration based on Ni-Cr and noble metal increased but the color of restoration based on gold sediment decreased.
    2-Year Clinic Effect of Fluoride Drop on Dental Caries Prevention of Primary Teeth
    LI Shao-min1, FANXu2, ZOU Jing1
    West China Journal of Stomatology. 2005, 23(02):  136-137. 
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    Objective To study the preventive effects of fluoride drop on dental caries for children.Methods The compara- tive method was carried out in 1 200 children aged from 3 to 4 .The subjectswere divided into three groups: fluoride drop group, fluoride toothpaste group and control group. The research period was 2 years.Results The results demonstrated that new dfswas 1.35±0.57 in the fluoride drop group after one year, 1.85±0.57 after two years. The new dfs was 1.53±1.09 in the fluoride toothpaste group after one year and 2.75±1.33 after two years. There was no significant difference of the dfs reduction between fluoride drop and toothpaste group.Conclusion Fluoride drop is effective in the prevention of dental caries in Chinese kinder- garten and primary school children.

    A Study on the Module Using Pendulum Combined with Straight Wire Appliance in Angle ClassⅡand Skeletal ClassⅠ Malocclusion
    ZHAOXiao-guang, LIUYue-hua
    West China Journal of Stomatology. 2005, 23(02):  138-141. 
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    Objective To investigate morphologic changes of Angle classⅡand skeletal classⅠpatients during pendulum and straight wire appliances treatment and to analyze the mechanism of this treatment protocol.Methods 10 patients with Angle classⅡand skeletal classⅠmalocclusion were recruited. The lateral cephalometric radiographs were evaluated to determine if there were significant differences among pre-treatment(T1), after molar distalization(T2), and post-treatment(T3) variables in- cluding skeletal, dental and soft tissue relationships.Results Differences between theT1 andT2 meanswere significant for distal movement and distal angulation of the maxillary molars (P<0·01), mesial angulation of the maxillary incisors (P<0·05), mesial movement of the maxilla (P<0·01). There were no significant changes betweenT1 andT3 in respect saggital position and angulation of the maxillarymolars (P>0·05). Onlythe mesial movement of lowerfirstmolars and upper and lower jawswere sig- nificant (P<0·01) duringthewhole treatment. No upper second molar impactionwas detected aftertreatment.Conclusion The upper molars distalization could be achieved by pendulum appliance. The classⅠmolar relationship could be obtained by the dif- ferential growth of jaws and the differential movement of upper and lower molars.

    Biological Effects of Nano-hydroxyapatite/Polyamide 66 on the Osteoblast
    YELing, SUQin, ZHOUXue-dong
    West China Journal of Stomatology. 2005, 23(02):  142-144. 
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    Objective To evaluate the biological effects of nano-hydroxyapatite/polyamide 66(nHA-PA66) on the growth and activity of osteoblast.Methods MTTassaywas used to determine the growth of osteoblast, enzymatic measure was used to deter- mine the activity of ALP and quantitative RT-PCR (QRT-PCR) to evaluate the changes of osteoclacin mRNA expression in os- teoblasts treated by DMEM eluate of nHA-PA66.Results Osteoblasts of different test groups demonstrated relative proliferation rate ranging from 98%~106% without dose-dependent effect. The ALP activity and osteocalcin mRNA expressionwere similar in test and control groups (P>0.05).Conclusion nHA-PA66 has no negative effects on the osteoblast and its osteoblast-compati- bility is proved.

    Osteogenic Differentiation of Synovial Mesenchymal Stem Cellsin vitro
    LI Jian1, LONG Xing1,ZHU Fan2, YANG Xue- chao3
    West China Journal of Stomatology. 2005, 23(02):  145-147. 
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    Objective To investigate the potential of synovial mesenchymal stem cells(SMSC) in osteogenic differentiation. Methods SMSCwere obtained by limited dilutionmethod and expanded to culture in 25-milliliterflasks. The attached cellswere treated with inductive medium containing dexamethasone, glycerophosphate and vitamin C at 3rd passage SMSC. The mineralized nodule was stained byVonKossa method. The expression ofALP and osteopontinwere detected by histochemical, immunohistolog- ical staining technique, respectively, while the expression of cbfa1 mRNAby RT-PCR.Results Pure SMSCwhichwere of spin- dle shape and star shape, uniform in size, could be induced to pleomorphism osteoblastin vitro, whichwere intensive positive in ALP and osteopontin. The expression of cbfa1 mRNAwere also verified by RT-PCR and the polygonal cells formed nodular struc- ture at 4 weeks. All these were coincidentwith the characters of osteoblast.Conclusion SMSC can be purified and induced into osteoblastin vitro.

    Cultivation and Induced Differentiation of Bone Marrow Stromal Cells of SD Rats with TypeⅠOsteoporosisin vitro
    TANG You-chao, TANG Wei, TIANWei-dong, CHENXi-zhe, LISheng-wei
    West China Journal of Stomatology. 2005, 23(02):  148-151. 
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    Objective To study the biological features and osteoblast/adipocyte phenotypes of bone marrow stromal cells (BMSCs) of Sprague-Dawley (SD) rats withTypeⅠosteoporosis by induced culture.Methods Six-month-old SD rats were used in this study.16 female rats were randomly divided into two groups. eight rats were ovariectomied as experimental group to estab- lish the modle ofTypeⅠosteoporosis, while other rats received sham-operation. Three month later BMSCs of 16 rats were isolated by discontinueous gradient centrifugation and then plated inα-MEM medium as primary culture. Secondary harvested cells were cultured for 14 days inα-MEMmedium supplemented with dexamethasone, ascorbic acid, vitamin D3,β-glycerophosphate or dex- amethasone, 3-isobutyl-1-methylxanthine, insuline, and indomethine. The cells were screened by inverted microscope each day and cell growth was studied with cell counting. The osteoblast and adipocyte phenotypes were verified by cytochemistry staining, counted the percentage of positive stained cells.Results The weight and bone mineral density of rats were statistically different between experimental group and control group. Gomori and Von Kossa′s staining demonstrated positive osteoblast phenotypes of al- kaline phosphatase and mineralized nods by osteogenic inducer, while Oil Red O staining identified BMSCs treatedwith adipogenic medium resulted in adipocyte formation and there was no significant difference in the percentage of positive stained cells between two groups.Conclusion The model of TypeⅠosteoporosis has been established successfully. BMSCs from SD rats with osteo- porosis maintain their differentiation potential.

    Study on Multi-Lineage Potential of Bone Marrow Mesenchymal Stem Cells Derived from Green Fluorescent Protein Transgenic Mice
    LI Zhi-yong, TIAN Wei-dong, LIULei, CHENXi-zhe, LIN Yun-feng, YAN Zheng-bin, CHENLing, LI Sheng-wei
    West China Journal of Stomatology. 2005, 23(02):  152-154. 
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    Objective To study the multi-lineage potential of bone marrow mesenchymal stem cells (MSCs) derived from transgenic mice with green fluorescent protein (GFP) genein vitro.Methods A6-week-old GFP transgenic mouse was executed by dislocation of cervical vertebra, and the marrow in tibia and thighbone was washed outwith asepsis. The limited cell strains of MSCs derived from GFP transgenic mice(GFP-MSCs)were obtained with density gradient centrifugation. The passage 3 GFP-MSCs were induced to differentiate into osteoblast, adippcyte, neuron with solution of calcium induction medium, adipogenic medium and neural induction medium respectively. After being calcium-induced, the activity of alkaline phosphatase on GFP-MSCs was determined by micro-plate reader, and alizarin red staining was performed to test the formation of calcium concentration. The adipo-induced MSCs were detected with oil red O staining. Immunocytochemical stainingwas performed to detect the expression of NSE on neuron-induced MSCs.Results The ALP activity of GFP-MSCs heightened gradually alongwith being calcium-induced, and alizarin red staining showed positive. Oil red O staining of adipo-induced cells and NSE immunocytochemical staining of neu- ron-induced cells demonstrated positive.Conclusion The limited cell strain of GFP-MSCs possessesmulti-lineage potential,which can be used as an efficient tracking facility for studying the mechanism of multi-lineage potential on the MSCs.

    The Expression of TGF-β1 mRNA in the Maxillary Sutures of Puberty Rhesus Loaded with the ClassⅢIntermaxillary Orthopedic Force
    WUTuo-jiang1, LISong2, XUYun2, LIHuang1, YINKang2
    West China Journal of Stomatology. 2005, 23(02):  155-158. 
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    Objective The purpose of the study is to detectthe expression ofTGF-β1 mRNA in the maxillary sutures of puberty rhesus during different periods of the loading of intermaxillary classⅢorthopedic force.Methods The animal model was estab- lished with 6 puberty female rhesus, whichwere randomly divided into experimental group(wearing classⅢtwin-blockmagnet ap- pliance, each 2 rhesus for 3 and 6 month respectively) and control group(not wearing any appliance, each 1 rhesus for 3 and 6 month respectively ). Tissue sections were obtained perpendicular to the sutures. In situ hybridization was used to the expression of TGF-β1 mRNA, and the expression intensitywasmeasured and statisticswas performed by SPSS 11.0.Results Therewere no statistic differences of cellular density between palatomaxillary suture in vertical group and pterygomaxillary suture in horizontal group, but statistic differences were found between other groups. The expression of TGF-β1 mRNA was detected in the control group, but the expression intensity was obviously increased after the load of intermaxillary classⅢorthopedic force. Statistically significant differences were found among all groups exceptthe sixmonths experimental group and control group of temporozygomatic sutures and pterygomaxillary sutures. Experimental groups were more intensive than the control group and three months group was more intensive than the six months group.Conclusion The active tissue remodeling happened in the circummaxillary sutures by the effect of classⅢintermaxillary orthopedic force. Cell proliferation, extracellularmatrix synthesis and bone formationwere ac- celerated. During the different remodeling periods, the expression intensities were different, which may be related to the different force loading manners, the different reaction of sutures tothe orthopedic force and the different biological function ofTGF-β1 in the different periods.

    Analysis of Chromosome Karyotype of Oral Carcinoma-associated Fibroblasts
    ZHENGXiao-hui1,LIUYing2,ZHOUHong- mei2,CHENQian-ming2,LI Bing-qi2
    West China Journal of Stomatology. 2005, 23(02):  159-160. 
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    Objective The purpose of this study was to investigate whether the fundamental genetic character of oral carcino- ma-associated fibroblasts changes through contrasting and analyzing the oral carcinoma-associated fibroblasts and the normal fibrob- lasts of oral mucosa.Methods The two kinds of cellswere treatedwith colchicine and microsometic fluid, and thenwere expand- ed with cold acetic acid and formalized with methyl alcohol. The cells were observed under the oil microscope after Giemsa stain- ing. The chromosome karyotype of the two kinds of cellswas analyzed by Visus 2.1.Results There were not obvious differences in the way of chromosome karyotype between the oral carcinoma-associated fibroblasts and the normal fibroblasts of oral mucosa. Conclusion The basic genetic characteristics of the normal cells are conserved in the oral carcinoma-associated fibroblasts, which means the cells have no malignant changes.
    The Integrinβ1 mRNA Changes after Orthodontic Movement of Teeth in Periodontitis Rats
    ZHANG Jing-ju1, CHEN Yang-xi2, XIAO Li-wei2, DUANPei-jia2, ZHAO Qing2
    West China Journal of Stomatology. 2005, 23(02):  161-163. 
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    Objective To study the integrinβ1 mRNA changes after orthodontic tooth movement in normal teeth and periodon- titis teeth of rats.Methods The OD of positively stained osteoclasts for integrinβ1 mRNAusing in situ hybridzationwas detected after orthodontic tooth movement in normal teeth and periodontitis teeth groups.Results Integrinβ1 mRNA expression were de- tected on all osteoclasts in toothmovement samples of normal and periodontitis teeth. There were stronger positive signals after giv- en orthodontic force in both of the two groups. But no differenceswere found after 0·5, 1, 2, 3, 5, 7, 10 days since orthodontic tooth movement. The integrinβ1 mRNAsignals in normal toothmovement groupwere not different fromthat in periodontitis group. Conclusion The integrinβ1 of osteoclasts may play a role in the stability and remodeling of periodontal ligament in orthodontic tooth movement. There were no difference in the OD of integrinβ1 mRNA staining in orthodontic tooth movement between normal teeth group and periodontitis teeth group.

    The Relationship of Abnormal Expression of Cell Glucoprotein with Recurrence of Pleomorphic Adenoma in Salivary Gland
    GAO Peng, ZHOUGeng-yin, SONGXiang-rong, HOUJian-xin, ZHANG Chui-juan, MAChao
    West China Journal of Stomatology. 2005, 23(02):  164-166. 
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    Objective To study the relationship of expression of mucin 1 and E-cadherin with recurrence of pleomorphic ade- noma in salivary gland, and to investigate the signal to predictthe recurrence potential of the tumor.Methods The capsule of tu- mor was observed bymicroscope. The expression ofmucin 1 and E-cadherin in 33 cases of primary adenoma, 12 cases of recurrent pleomorphic adenomas and 7 cases of malignant pleomorphic adenomas were detected by immunohistochemistry.Results There was no significant difference about the status of capsule and the positive rate of mucin 1 expression between primary and recurrent pleomorphic adenoma (P>0·05). The abnormal distribution of mucin 1 expressionwas observed in recurrent pleomorphic adeno- ma (6/8), whichwas characterized by the positive staining of the whole cytomembrane. On the other hand,positive staining of the primary pleomorphic adenomawas observed on the top of the membrane (19/21). The differencewas statistically significant (P< 0·05). The staining pattern in malignant pleomorphic adenoma was similar with the recurrent ones except higher ratio of positive expression .No significant differentwas observed among the three kind of tumors on the expression rate of E-cadherin(P>0·05). Conclusion The status of capsule didn′t have much actual usage in predicting the recurrence of pleomorphic adenoma. There was no significant relationship between the expression of E-cd and the recurrence of the tumor. The abnormal distribution of mucin 1 expression contributes to the invasiveness of the tumor and can be used as the predictive signal for recurrence of pleomorphic adenoma.

    The Study on Three-dimension Finite Element Analysis of the Stress Distribution in the Mandible Bone around Dental Implants
    WANGXiao-jie, LUO Jiao-ming, CHENJi-yong, ZHANG Xing-dong
    West China Journal of Stomatology. 2005, 23(02):  167-169. 
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    Objective The aim of this studywas to investigate howto model an accuracy 3-dimension Finite Element Analysis (3D-FEA) model.Methods Based on computed tomography (CT) scan data of a woman, a 3D finite element model of the first molar on the left was rebuilt by computer imagines process and computer aided design (CAD). Analysis of the stress distribution on a cylinder dental implant and in the bone around itwas conducted.Results The stress distribution showed extremely asymme- try in bucco-lingual section: stress concentrated on the lingual side of the mandible; stress mainlywas tensile in buccal side, and on the contrary compressive in lingual side.Conclusion The resultswere more reliable because this model more really displayed the mandible.

    Expression and Secretion of Human Tumor Necrosis Factor Gene Transfected on Human Embryo Myoblasts
    GAO Zhen- nan, GAO Jia-rang, TIAN Wei-dong, LI Sheng-wei, LIULei, FU Chun-hua
    West China Journal of Stomatology. 2005, 23(02):  170-172. 
    Abstract ( 961 )   HTML ( 0 )   PDF(pc) (167KB) ( 332 )   Save
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    Objective To observe human tumor necrosis factor-α(hTNF-α) expression and secreting level of human embryo myoblasts transfected by hTNF-αgene.Methods Human embryo myoblasts were transfected with shuttle plasmid pSV23SHTNF containing hTNF-αgene by cationic liposomes DOSPER. The control group was only given equivalent liposomes except plasmid. After culturing for 24, 48, 72 and 96 hours, hTNF-αexpression level of human embryo myoblastswas observed with immunocyto- chemistry staining, and hTNF-αsecreting of human embryo myoblasts was analyzed by ELISA.Results After transfected by hT- NF-αgene for 24 , 48, 72 and 96 hours, the human embryomyoblasts displayed significant secretion of hTNF-αin the cultural su- pernatant(P<0.05), and overexprsssion in cytoplasma and cell membrane.Conclusion Transfection of hTNF-αgene to human myoblasts made myoblasts secrete high concentration of hTNF-α, implying it is feasible that transfecting muscle cells surrounding tongue carcinoma lesion with hTNF-αgene can prevent tongue carcinoma from intruding into deeper muscle tissue.

    The Malocclusion of Primary Dentition in the Suburb of Chengdu: a Cross-section Survey
    HUANG Ning1, SHI Zong- dao1, WANG Zu-hua2, QINJun-cheng2, CHEN E1, GUO Chun-lan1, GUO Hong-liang2
    West China Journal of Stomatology. 2005, 23(02):  173-174. 
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    Objective To reveal malocclusion of primary dentition in the suburb of Chengdu.Methods Cross-section survey and malocclusion rate calculation were performed.Results There are 491 children with malocclusion from total 1 279 children; the rate of malocclusion is 38.38%.The most important malocclnsion in primary dentition are over bite(Ⅲ°),anterior cross bite, edge to edge bite,early loss of deciduous teeth, fused teeth.Conclusion The rate of malocclusion of primary dentition in the suburb of Chengdu is a little bit ligh.Much work should be done to prevent or treat the malocclusion of primary dentition.

    Analysis of the Literature in West China Journal of Stomatology Cited by Science Periodical Embodied by Chinese Biological Medical Disc
    WANG Qing, WUAi-hua
    West China Journal of Stomatology. 2005, 23(02):  175-176. 
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    Objective The literature published in West China Journal of Stomatology (WCJS) cited by science periodical from 1983 to 2001 were analyzed to evaluate the academic level and quality of WCJS.Methods The cited literature was searched by Chinese biological medical disc (CBMDisc) from 1983 to 2001. The distributions of the times, the periodical and the authorswere described.Results The quantities of cited literature were 1 338 and increased with years. The periodical and the authors were 266 and 1 003 respectively from1998 to 2001. The cited frequencies of stomatological periodicalswere more than that of other aca- demic periodical. The authors scattered in 32 provinces and 448 units of China. The medical university and affiliated hospitalwere 32·14%, the local hospital and stomatological dispensary were 54·69%, the military hospital was 10·71%, and other unit was 2·46%.Conclusion The literature published in WCJS are superior in quality. The cited frequency of WCJS is high relatively. WCJS is one of the most important informational source of stomatology.