Objective To investigate the change of T lymphocyte subsets, interleukin（IL）-2 and tumor necrosis factor（TNF）-α in the tumor- bearing mice and patients with oral cancer receiving thermo-chemotherapy, and investigate the correlation among them. Methods After treatments, the expression of lymphocyte transformation index（LTI）, IL-2 and TNF-α in the tumor-bearing mice were detected with methyl thiazolyl tetrazolium（MTT）, the expression of IL-2 and TNF-α in the patients with oral cancer were detected with MTT, the expression of LTI, CD4+ and CD8+ were detected with 3H-TdR incorporation. Results LTI, IL-2 and TNF-α of thermo-chemotherapy group（HP group） had no significant difference comparing with those of normal mice group（N group）（P>0.05）, but which were significantly
higher than those of chemotherapy group（P group） and no treatment group（NT group）（P<0.01）. In clinical trials, the expression of LTI, CD4+, CD4+/CD8+, IL-2 and TNF-α on oral cancer patients after thermo-chemotherapy were significantly higher than those before thermo-chemotherapy（P<0.01）. Conclusion After thermo-chemotherapy, the expression of LTI, IL-2 and TNF-α of tumor-bearing hosts are significantly improved, there is a significant correlation between IL-2 and T cell.