Abstract:

Objective　The aim of this study was to explore the expression of basic fibroblast growth factor (bFGF) and fi- bronectin during the healing process of allografttissue engineering skin.Methods　The tissue engineering skin thatwas obtained from neonatal SD rats was cultured in the lab. Afterwards, the skin was grafted into adult SD rats, and the expressions of bFGF and fi- bronectin were detected on the 7th, 10th,14th, 20th and 30th day after the allograft of the tissue engineering skin. The autografted skin in 15 adult Wistar rats and the normal skin in 15 Wistarwere treated as the control. HE staining and immunohistochemical stain- ing were used to examine the healing of grafted skin.Results　The expression of bFGF and fibronectin was the strongest on the 10th day aftergraft, andwasweaker before the 10th day and afterthe 14th day. The expression changes of bFGF and fibronectinwere sim- ilar as they were in the autograft group.Conclusion　The expression changes of bFGF and fibronectin in the tissue engineering skin during the process of wound healing were similar to those of autografted skin, and these can promote the repair of tissue engineering skin allograft.

Key words: skin, basic fibroblast growth factor, fibronection, allograft