West China Journal of Stomatology ›› 2021, Vol. 39 ›› Issue (3): 274-278.doi: 10.7518/hxkq.2021.03.005

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Expressions of cytokeratin 14 and proliferating cell nuclear antigen in the Hertwig’s epithelial root sheath of a Vps4b knockout mouse

Tian Qing1(), Wang Yingying2, Li Qiang1, Chen Dong1()   

  1. 1.Dept. of Stomatology, The First Affiliated Hospital of Zhengzhou University, Henan Provincial Stomatological Hospital, Zhengzhou 450052, China
    2.Dept. of Stomatology, Xuzhou Central Hospital, Xuzhou 221009, China
  • Received:2020-05-26 Revised:2020-12-19 Online:2021-06-01 Published:2021-05-26
  • Contact: Chen Dong E-mail:635448763@qq.com;fccchend@zzu.edu.cn
  • Supported by:
    Key Research and Development Project of Henan Province(22170124);Medical Science and Technique Project of Henan Province(SBGJ2018038)

Abstract: Objective

The effect of Vps4b gene mutation on the expressions of cytokeratin 14 (CK14) and proliferating cell nuclear antigen (PCNA) in the Hertwig's epithelial root sheath (HERS) is investigated.

Methods

The bilateral mandibular tissues of mouse on postnatal days 5, 9, 11, 15, and 19 were removed. The mandibular first molar tissue sections were obtained after paraffin embedding. The CK14 and PCNA expressions in the epithelial root sheath of the normal mouse and Vps4b knockout mouse were compared through immunohistochemistry.

Results

On postnatal day 5, the normal mouse began to form HERS and had a strong positive PCNA expression in the HERS cells; on postnatal day 9, the HERS structure was continuous, and PCNA was positive in the HERS cells; on postnatal day 11, a small portion of HERS began to break, and PCNA was weakly positive in the HERS cells; on postnatal day 15, HERS continued to fracture; PCNA was weakly and positively expressed in the HERS cells on the root surface; on postnatal day 19, the tooth root reached normal physiological length, and PCNA was positively expressed in the HERS cells of the terminal part. Similar to the normal mouse, the gene knockout mouse also formed a HERS structure on postnatal day 5. However, HERS began to break on postnatal day 9. On postnatal day 19, only a few fragments of HERS were found on the root surface, and the root development was immature. Moreover, the expression intensity of PCNA in the gene knockout mouse was decreased.

Conclusion

The Vps4b gene mutation may change the CK14 and PCNA expressions, leading to abnormal root development.

Key words: Vps4b gene, Hertwig's epithelial root sheath, cytokeratin 14, proliferating cell nuclear antigen, gene knockout

CLC Number: