West China Journal of Stomatology ›› 2018, Vol. 36 ›› Issue (3): 240-246.doi: 10.7518/hxkq.2018.03.002

Previous Articles     Next Articles

Preparation of multifunctional nanoparticles targeting tongue cancer and in vitro study

Wei Ren1(), Lihua Qiu1,2(), Zhi Gao3, Pan Li4, Xin Zhao5, Chengchen Hu1   

  1. 1. Dep. of Oral and Maxillofacial Surgery, Stomatological Hospital of Chongqing Medical University, Chongqing Key Laboratory of Oral Diseases and Biomedical Science, Chongqing 401147, China
    2.Chongqing Municipal Key Laboratory of Oral Biomedical Engineering of Higher Education, Chongqing 401147, China
    3. Dept. of Stomatology, The Second Affiliated Hospital of Chongqing Medical University, Chongqing 400016, China
    4. Institute of Ultrasound Imaging, Chongqing Medical University, Chongqing 400010, China
    5. Chongqing Collaborative Innovation Center for Functional Food, Chongqing University of Education, Chongqing 400067, China
  • Received:2017-11-14 Revised:2018-03-28 Online:2018-06-01 Published:2018-06-01
  • Supported by:
    Chongqing Science and Technology Innovation Projects of Social Livelihood (cstc2016shmszx00010);;Program for Innovation Team Building at Institutions of Higher Education in Chongqing in 2016(CXTDG201602006);;Chongqing Municipal Key Laboratory of Oral Biomedical Engineering of Higher Education [(2014)55].


Objective This study aims to prepare docetaxel (DOC)-loaded multifunctional nanoparticles containing indo-cyanine green (ICG) and perfluorohexane (PFH) as targeted drug delivery system, which is supplemented with stromal cell-derived factor-1 (SDF-1), and characterize their properties. Methods Multifunctional nanoparticles were prepared by using the double emulsion method. SDF-1 was covalently conjugated to the surface of the nanoparticles through thioe-ther bonding. Their particle size, distribution, and surface potential were determined with the Malvern measuring in-strument. The conjugation of SDF-1 was evaluated by confocal laser scanning microscope. Encapsulation efficiency (ELC), drug loading capacity (DLC), and release regularity of the nanoparticles were determined by high-performance liquid chro-matography (HPLC). In vitro photothermal property was recorded by a thermal imager. The in vitro imaging capacity was observed by a photoacoustic instrument and an ultrasonic diagnostic apparatus. Targeting capability was assessed by flow cytometry. The cell activity on SCC-15 cells was checked by CCK-8 method. Results The targeted multifunctional nano-particles showed regularly sphericity. The diameter was (502.88±17.92) nm. The zeta potential was (-11.5±3.15) mV. ELC was 54.12%±1.74%. DLC was 1.08 mg·mL-1. In vitro drug release was initially fast and subsequently slow. The photothermal characteristics were related to the concentration; the higher the concentration, the higher the temperature. Nanoparticles could detect significant photoacoustic and ultrasound signals. The in vitro targeting rate was 89.99%. No significant differences of cell viability in the SINPs groups were observed at each concentration (P>0.05). The inhibition effect of DOC-SINPs was stronger than that of SINPs whether or not in the presence of laser irradiation among the groups of 150 and 200 μg·mL-1 (P<0.05). Conclusion Multifunctional nanoparticles for diagnosis and treatment were successfully prepared and displayed dual-mode ultrasound/photoacoustic imaging and antitumor effects of chemotherapy and photothermal therapy.

Key words: poly (lactic-co-glycolic acid), indocyanine green, targeted therapy, tongue carcinoma, molecular imaging

CLC Number: