### Effect of MT01/PEN complexes on the expression of osteoprotegerin and receptor activator of nuclear factor κB ligand in human osteoblast-like cell line MG63

Cui Ye1,2, Zheng Yi3, Shen Yuqin3, Hou Xu1, Lou Yixin1, Sun Xinhua1

1. 1. Dept. of Orthodontics, School and Hospital of Stomatology, Jilin University, Changchun 130021, China; 2. Jilin Provincial Key Laboratory of Tooth Development and Bone Remodel, Changchun 130021, China; 3. Dept. of Periodontology, School and Hospital of Stomatology, Jilin University, Changchun 130021, China
• Online:2016-02-01 Published:2016-02-01

Abstract:

Objective  This study aims to synthesize MT01 (a kind of oligodeoxynucleotides) and N-isopropylacrylamidemodified polyethylenimines (PEN) complexes (MT01/PEN) as well as to investigate the effect of the complexes on the expression of osteoprotegerin (OPG) and the receptor activator of nuclear factor κB ligand (RANKL) in the human osteoblast-like cell line MG63. Methods  MG63 cells were transfected by MT01/PEN complexes formed with three different mass ratios (1∶2, 1∶4, 1∶6) of MT01 to PEN. MT01 and MT01-s were used as positive control. Enzyme-linked immunosorbent assay and real-time polymerase chain reaction were performed to estimate the amount of OPG and RANKL released into the culture media and in MG63 at 24, 48, 72 h. Results  MG63 responded to the MT01/PEN complexes by significantly upregulating the OPG on the protein and mRNA levels (P<0.05). The protein and mRNA levels of RANKL were lower in most of the groups with complexes, and the OPG/RANKL ratio were higher (P<0.05). MG63 were affected by the MT01/PEN complexes with different mass ratios, particularly when the ratio was 1∶6. Conclusion  MT01 can enhance the promotion of ossification by establishing the delivery system with PEN.