Abstract:

Objective To study the drug resistance changes in Tca8113 cell lines by exposing to carboplatin. Methods The concentration of carboplatin added to Tca8113 cells was increased gradually and continually, which was to induce the carboplatin- resistance in Tca8113 cells. The sensibility to drugs of the cells was analyzed by MTT method. Immunocytochemistry and RT- PCR were utilized to examine the expression of multidrug resistance proteins and genes. Results After exposing to carboplatin, the Tca8113/CBP cells had higher drug- resistance to CBP, MTX, PYM, VCR and higher expression of MRP, GST - π than Tca8113 cells. Conclusion Multidrug resistance of Tca8113/CBP is associated with over expression of MRP, GST- π and MDR. Tca8113/CBP can provide an ideal model for multidrug resistance research.

Key words: Tca8113 cell line, multidrug resistance, carboplatin