Abstract:

Objective To relatively quanti~ the gene expression of fatty acid synthase in squamous cell carcinoma, adjacent tissue，and some normal oral tissues by real-time quantitative PCR．Methods The tissues were collected flesh from surgical specimens．The collected tissues were minced．Then the total RNA was extracted． The RNA was reversely transcripted into cDNA with random prime．And then the cDNA was amplified by real-time quantitative PCR to quantify the gene expression of FAS according to an internal control GAPDH．The diference of FAS gene expression Was compared between squamous cell carcinoma，adjacent tissue，and some normal oral tissues．Results The expression of FAS of squamous cell carcinoma Was notably higher than the other two(P<0．o01)．Conclusion Real-time quantitative PCR provides a method for monitoring the expression of fatty acid synthetic activity in squa- mous cell carcinoma，adjacent and normal tissues．

Key words: squamous cell carcinoma of oral cavity, real-time quantitative PCR, fatty acid synthase