Abstract:

Objective:'The aim of this study was to establish a combined culture model of A/J mouse embryonic palatal shelves mesenchymal and epithelial cells (palatal cells)，and study their biological behavior in vitro. Methods: The A/J mouse embryonic palata cells were harvested from 13-day-old A/J mouse fetus by dissection and sequential digestion with 0.25 % trypsin. The isolated cells were cultured in DMEM/F-12 medium and observed by phase-contrast而croscope, flow cytometer, cytoskeleton staining and PGE2-RIA. Results: The embryonic palatal mesenchymal and epithelial cells grew well and, their morphology and cytoskeleton did not change in vitro. The cells were full of abundant protein and DNA, and had strong ability to metabolize and proliferate Conclusion: The mesenchymal and epithelial cells of A/J mouse embryonic palatal shelves in this combined culture system can keep their basic biological behavior.

Key words: cell culture, embryonic palatal shelves, A/J mouse