Objective  To examine the relationship between NF-κB signal transduction way and the sensitivity of Tca8113 carcinoma cell to Pingyangmycin chemotherapy.

Methods  2 mg/L antisense p65 oligodeoxynucle-otides（5′-GAACAGTTCGTCCATGGCCG-3′） was transfected into Tca8113 cells through Lepofectin vectin, then the cells were treated with 8 mg/L Pingyangmycin. After 3 h and 6 h, the expression of p65 in nucleus was studied with im-munohistochemical methods and Western blot analyses. After 48 h, the inhibitory rate of cell growth was detected with MTT assay. Results  The NF-κB/p65 signal  transduction way in Tca8113 cells was activated after treated  with Pingyangmycin. But the transfection of antisense p65 oligodeoxynucle-otides inhibited the activation of  the sig- nal transduction, the p65 expression in nucleus was decreased significantly（P<0.05） at 6 h, and the inhibitory rate of cell growth was increased significantly（P<0.05） at 48 h. Conclusion  The results suggested the chemotherapeutic sensitivity of Tca8113 carcinoma cell to Pingyangmycin was improved by the inhibition of NF-κB/p65 signal transduction.