Abstract:

Objective　To studythe therapeutic effects of combined gene therapy ofwild type p53 (wt-p53) and herpes simplex virus thymidine kinase (HSV-tk) gene on pleomorphic adenoma cells of salivary gland.Methods　Wild type p53 and HSV-tk gene were transfected into human pleomorphic adenoma cells of salivary gland by using recombinant adenovirus vector. The effi- ciency of transfection was checked and gene was expressed by RT-PCR methods. The cell inhibition after transfected was verified by light microscope and MTT.Results　The proliferation of the pleomorphic adenoma cells transfected wt-p53 and HSV-tk gene was inhibited and the cell survival rate decreased to 54% and 38% respectively in 5 days. However, whenwt-p53 gene combined withHSV-tk/GCV system, the killing effects was significantly stronger (P<0.05) and the cell survival rate decreased to 20%. Conclusion　Combining p53 gene withHSV-tk/GCV system for gene therapy in pleomorphic adenoma cells of salivary gland is a valuable method.

Key words: adenoma, salivary gland, gene therapy, wild type p53, herpes simplex virus thymidine kinase gene