West China Journal of Stomatology ›› 2021, Vol. 39 ›› Issue (4): 434-440.doi: 10.7518/hxkq.2021.04.009

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Expression of Ki-67, Cyclin D1, P53, and P16 in patients with oral leukoplakia and leukoplakia cancerization with spicy diet in Chengdu

Wan Zixin(), Zheng Zhijian, Huang Meichang, Chen Yu, Yao Lihong()   

  1. State Key Laboratory of Oral Diseases & National Clinical Research Center for Oral Diseases & Dept. of Oral Pathology, West China Hospital of Stomatology, Sichuan University, Chengdu 610041, China
  • Received:2020-05-06 Revised:2021-03-16 Online:2021-08-01 Published:2021-08-10
  • Contact: Yao Lihong E-mail:wanzixinhxkq@126.com;yaolihongsansan@126.com
  • Supported by:
    Clinical Research Project of West China Hospital of Stomatology of Sichuan University(LCYJ2019-8)

Abstract: Objective

To investigate the expression of Ki-67, Cyclin D1, P53, and P16 in patients with oral leukoplakia (OLK) and OLK cancerization who have aspicy diet in Chengdu.

Methods

Thirtypatients with OLK andspicy diet and 15 patients with OLK without spicy diet in Chengdu were divided into three groups: hyperplastic OLK (OLK-), OLK with mild to moderate dysplasia (OLK+), and severe dysplastic  OLK or oral squamous cell carcinoma (OSCC) transforming from OLK (OLK++/OSCC). The expression of Ki-67, Cyclin D1, P53, and P16 were detected by immunohistochemistry and statistically analyzed.

Results

The expression of Ki-67 and P53 in patients with or without spicy diet in the OLK+and OLK++/OSCC groups were stronger than that of the OLK- group (P<0.05). The OLK++/OSCC group showed a higher expression of Cyclin D1 and lower expression of P16 than the OLK- group (P<0.05). The expression of Ki-67, Cyclin D1, P53, and P16 in patients with spicy diet and without spicy diet had no substantial difference. The expression of Ki-67 and Cyclin D1 showed a positive correlation (r=0.439, P=0.015).

Conclusion

Spicy diet did not have an influence on the expression of Ki-67, Cyclin D1, P53, and P16 in patients with OLK and OSCC. The expression of Ki-67, Cyclin D1, and P53 increased with the development of OLK, whereas P16 showed opposite expression trend.

Key words: spicy diet, oral leukoplakia, cancerization, oral potentially malignant disorders

CLC Number: