West China Journal of Stomatology ›› 2021, Vol. 39 ›› Issue (4): 413-418.doi: 10.7518/hxkq.2021.04.006

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Effect of piceatannol against malignant melanoma in vivo and in vitro

Yu Bo(), Liu Wei, Hu Minqi, Tang Xiufa, Li Chunjie, Que Lin()   

  1. State Key Laboratory of Oral Diseases & National Clinical Research Center for Oral Diseases & Dept. of Head and Neck Oncology, West China Hospital of Stomatology, Sichuan University, Chengdu 610041, China
  • Received:2020-08-05 Revised:2021-05-07 Online:2021-08-01 Published:2021-08-10
  • Contact: Que Lin E-mail:1005137874@qq.com;jy959896@163.com
  • Supported by:
    The National Natural Science Foundation of China(81902775);Sichuan University Innovation Spark Project(2018SCUH0055)

Abstract: Objective

To study the antitumor effect of piceatannol (PIC) on malignant melanoma in vitro and in vivo.


B16F10 cells were cultured in vitro and treated with gradient concentrations of PIC. Cell viability was detected with methyl thiazolyl tetrazolium (MTT) assay; matrix metalloproteinase (MMP)-2, MMP-9, vascular endothelial growth factor (VEGF), spleen tyrosine kinase (Syk), and p-Syk were detected with Western blot; migration ability was detected with wound healing assay; invasion ability was detected with Transwell assay. Syk expression was suppressed through RNA interference for the detection of the possible mechanism of PIC in melanoma. An in vivo study was established by creating B16F10-bearing mice with intraperitoneal injection of PIC.


The cell viability of B16F10 decreased with increasing PIC concentration. The results of the Transwell assay showed that invasion ability decreased with increasing PIC concentration, and healing time was prolonged at increased PIC concentration in the wound healing assay. Western blot results showed that PIC mainly inhibited the phosphorylation of Syk and inhibited the expression of MMP-2, MMP-9, and VEGF. RNA interference pointed out that blocking the expression of Syk can reveal the same inhibition effect on B16F10 cells as PIC. In vivo study revealed that different concentrations of PIC cangreatly inhibit melanoma progression.


PIC might block the progression of malignant melanoma by inhibiting spleen tyrosine kinase.

Key words: piceatannol, spleen tyrosine kinase, malignant melanoma

CLC Number: