West China Journal of Stomatology

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The effect of ginkgolic acids on multidrug resistance in oral squamous cell carcinoma

WANG Hui, ZHOU Chen-chen, FENG Yun, DAI Li-na, CHEN Jiao, CHEN Si-xiu, LI Xiao-yu, LIU Yu-rong, ZHANG Ping   

  1. State Key Laboratory of Oral Diseases, Sichuan University, Chengdu 610041, China
  • Received:2010-12-25 Revised:2010-12-25 Online:2010-12-20 Published:2010-12-20
  • Contact: ZHANG Ping,Tel:028-85503495

Abstract:

Objective Carboplatin(CBP)-resistant cell line(Tca8113/CBP) and pingyangmycin(PYM)-resistant cell line(Tca8113/PYM) were established in vitro. Ginkgolic acids’influence over multidrug resistance(MDR) of drug-resistant cells was discussed by ginkgolic acids coupled with chemotherapy drugs. Methods The expression of P-glycoprotein(P-gp) was detected by immunohistochemistry. MTT assay was applied to ascertain the resistance index of drug-resistant cells. The effect of different concentrations of ginkgolic acids on the proliferation of drug-resistant cells and parental cell was measured by MTT assay. Making sure the non-toxic concentration of ginkgolic acids and observing the reversal effect of ginkgolic acids on drug-resistant cells. Resistance index was redetermined by MTT assay after ginkgolic acids coupled with chemotherapy drugs induced the cell lines for some time. Results Immunohistochemistry showed that P-gp positive expression rate of drug-resistant cells was significantly higher than parental cells. The non-toxic concentration of ginkgolic acids which was determined by MTT assay was 10 μg·mL-1. The reversal folds of Tca8113/CBP cell line to CBP and Tca8113/PYM cell line to PYM were 2.94 and 2.43 respectively. Before coupled with ginkgolic acids, the resistance indices of Tca8113/CBP and Tca8113/PYM cell lines were 3.24 and 11.9 respectively. When ginkgolic acids was added with chemotherapy drugs for some time, the resistance indices of Tca8113/CBP and Tca8113/PYM cell lines were 2.18 and 4.43 respectively. Conclusion This experiment successfully induced the drug-resistant cell lines of Tca8113/CBP and Tca8113/PYM. The method of chemotherapy drugs coupled with ginkgolic acids further confirmed the effect on proliferation of Tca8113/CBP and Tca8113/PYM cell lines was reducing. Non-toxic concentration of ginkgolic acids can partially reverse the drug resistance of Tca8113/ CBP and Tca8113/PYM cell lines. Furthermore, MDR level of drug-resistant cells decreased somewhat when they were induced by ginkgolic acids coupled with chemotherapy drugs for some time.

Key words: multidrug resistance, P -glycoprotein, ginkgolic acids