West China Journal of Stomatology

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Observation on osteoblasts responded to fluid induced shear cultured on different surfaces with scanning electron microscope

LI Wen-hui1,2, YANG Xiao-yu3, XIAN Su-qin1   

  1. 1. State Key Laboratory of Oral Diseases, Sichuan University, Chengdu 610041, China; 2. Dept. of Stomatology, 416 Hospital of Nuclear Industry, Chengdu 610041, China; 3. Dept. of Implantology, Stomatology Hospital of Guangdong Province, Guangzhou 510280, China
  • Received:2010-12-25 Revised:2010-12-25 Online:2010-12-20 Published:2010-12-20
  • Contact: XIAN Su-qin,Tel:028-85502141


Objective To investigate morphological change of osteoblasts cultured on titanium plates with different microarchitecture structure when exposured to fluid shear stress. Methods 14 dynes·cm-2 fluid shear stress was applied on osteoblasts cultured on 3 different commercially pure titanium plates: Polished treatment(PT), sandblast (SB), sandblasting and acid-base(SB-AB) surfaces. Scanning electron microscope(SEM) was adopted to observe the morphological changes after 0.5, 4, 7.5 h time point respectively. Results Morphologically, no significant changes were observed after 0.5 h and few osteoblasts were seen after 7.5 h on all 3 type of different surfaces, and significant changes could only be observed after 4 h. Osteoblasts were elongated and rearranged along the flow way on different levels on PT surface. Shape of cells was altered, from long fusiform suspending over depressed areas into polygon stretching out many synapsises tightly attachedto pits on SB-AB surface. Osteoblasts on SB surface displayed similar change as SB-AB surface, besides, some cells were elongated along the way of flow, stretching out threadlike synapsises attached to edges of pits. Conclusion Morphological change of osteoblast responding to fluid shear stress in physiological range depends on substrate microarchitecture and varies with the time of fluid shear stress application.

Key words: fluid shear stress, microarchitecture, osteoblasts