Foreign body aspiration and ingestion during oral disease treatment are possible to occur. The incidence is prone to take place among children. Extracted teeth, endodontic instruments, prosthesis, and orthodontic appliance items are possible to be aspirated or swallowed. This article summarizes the reasons, handling methods, and prevention of foreign body aspiration and ingestion to provide guidance for clinical work.

Objective  This study investigated the effects of systemic administration of oxytocin (OT) in osteoporotic rats on implant osseointegration. Methods  Twenty rats were randomly assigned to the control and experimental groups. Initially, the rats underwent bilateral ovariectomy. After 12 weeks, an osteoporosis model was established. Each rat received an implant at the distal and middle femoral metaphysis. Simultaneously, systemic administration was conducted with one group receiving subcutaneous injection of OT (1 mg•kg−1 per day), whereas the other group received placebo injection. After treatment for 4 weeks, another surgery was conducted to remove the thigh bones from the rats containing the implants for an eight-week observation. With the employment of micro-CT, histological observation and push-out test, osseointegration was evaluated. While the rats received thigh-bone removal surgery, another surgery was conducted to remove the tibia metaphysis from the rats of both groups to perform histological observation and micro-CT inspection. Results  The trabecular bone of tibial samples was intensive and formed woven mesh structure in the experimental group compared with the control group. In the experimental group, the relative bone volume/tissue volume surrounding the implant, the bone contact ratio, and the maximum push-out force of the implant were 0.35%±0.06%, 67.25%±9.06%, and (70.32±10.91) N, respectively, the corresponding values were 0.11%±0.02%, 43.25%±7.01% and (21.65±4.36) N in the control group, and the experimental group increased significantly compared with the control group (P<0.05). Conclusion  Systemic administration of OT cannot only antagonize the negative effects of osteoporosis but can also promote implant healing and osseointegration of pure titanium implants.

Objective  This study investigated the effects of strontium ranelate (SrR) on the rats’ palatal suture after rapid maxillary expansion (RME). Methods Thirty-six male 6-week-old Wistar rats were randomly divided into three groups: control group (A), expansion only group (B), and expansion plus SrR group (C). Each group comprised 12 rats. Neither expansion nor SrR was given to group A. Each rat in groups B and C was set an orthodontic appliance with an initial expansive force of 1 N. The rats in group C were administered with SrR (600 mg•kg−1 body weight) at the same time every day after RME. All the rats were then euthanized in batches on days 4, 7, and 10. The width of the rats’ upper jaw was measured. Histological observation was used to section the rats and count the osteoblasts. Results  After the RME, no statistical difference was observed on the rats’ upper jaw width in group A (P>0.05). However, the change of upper jaw width in groups B and C presented a statistical significance (P<0.05). By contrast, no statistical difference was observed between groups B and C (P>0.05). The rats’sections were placed under a microscope, and some red fibrous tissues, mesenchymal cells, chondrocytes, and osteoblasts were observed in group A. More red fibrous tissues, mesenchymal cells, and chondrocytes were observed in groups B and C. In addition, more osteoblasts were observed on the edge of mid-palatal suture of the rats. Group C contains more osteoblasts than group B. Conclusion  RME can expand the mid-palatal suture of rats, which were in the growth development period, and increase the width of dental arch. SrR may promote osteoblast differentiation and hasten new bone formation in the expanded mid-palatal suture. Both conditions accelerate new bone formation and bone deposition calcification, which may be therapeutically beneficial to prevent relapse after RME.

Objective  To observe the expression of wingless-type MMTV integration site family, member 5A (Wnt5A)/ receptor tyrosine kinase-like orphan receptor 2 (Ror2) signal in the dental follicle cells during the normal eruption of the teeth as well as to explore the relationship between the expression of dental follicle cells and the formation of mature osteoclasts and eruption of the teeth. Methods   The mandibulars of 1–13 d old SD rats were separated to observe the growth and development of the teeth and alveolar bone through hematoxylin-eosin(HE) staining. Ror2 and Wnt5A expressions in rat dental follicle were also observed through immunohistochemistry. Dental follicle cells from the lower first intact molar germs of 5-6-day old SD rats were separated and cultured. Results  On the second day after birth, the dental follicle began to differentiate into periodontal tissues, but no obvious changes were observed in the alveolar bone one to three days after birth. On the fourth day, the number of osteoclasts increased significantly. The results of immunohistochemistry showed that Wnt5A was not significantly expressed in rat dental follicle tissues before the fourth day, but positive expression was expressed in the next day and continued to express to thirteenth days. Ror2 was expressed in the rat dental follicle at postnatal days 1–3, but weak expression was found in days 4–13. Conclusion  Wnt5A and Ror2 expressions in the process of tooth eruption have specific time distributions, suggesting that these expressions may participate in the regulation of the eruption of the teeth.

Objective This study aims to investigate the gene mutational characteristics of cathepsin C (CTSC) gene in a Chinese patient with Papillon-Lefèvre syndrome (PLS), then further confirm the genetic basis for the phenotype of PLS, and obtain genetic information that can be used as guide in the diagnosis and treatment of PLS. Methods  With their consent, peripheral blood samples were obtained from the proband and his family members (his parents and older sister) for genomic DNA extraction. The coding region and exon/intron boundaries of the CTSC gene were amplified and sequenced using polymerase chain reaction and direct sequencing of DNA. Results  Compound heterozygous mutations of CTSC gene were identified in the patient. The proband carries one heterozygous nonsense mutation c.754C>T in exon 5 and one heterozygous missense mutation c.1040A>G in exon 7. Both parents were heterozygous carriers without the clinical symptoms of PLS. None of the mutations were detected in the proband’s sister. Conclusion  The study proves that mutations of CTSC gene are responsible for the phenotype of Papillon-Lefèvre syndrome.

Objective  This study investigated the shear bond strength and the ability of resin adhesive containing different amounts of bioactive glass (BG) to prevent dentin demineralization. Methods  BG bonds at 30%, 40%, and 50% concentrations comprised a mixture of coupling agents modifying BG and Single Bond 2. Forty permanent premolars extracted for orthodontic treatment were randomly divided into four groups, namely, control group (BG-free), 30% BG group, 40% BG group, and 50% BG group. Composite resin and dentin were prepared into bonding specimens by using respective adhesives and then immersed in artificial saliva at pH 4 for 1 week. Changes in the pH values of the solution were measured every 24 h. The microtensile bond strength (TBS) of each specimen was examined after immersion in distilled water at 37 °C for 24 h. Results  The TBS values of the control group, 30% BG group, 40% BG group, and 50% BG group were (27.76±0.29), (27.05±0.58), (26.95±0.69), and (25.12±0.24) MPa, respectively. The TBS of the control group was not significantly different from that of the 30% BG group (P=0.06) but significantly higher than that of the 40% BG and 50% BG groups (P=0.04 and P=0.00, respectively). In addition, TBS was significantly higher in the 30% BG and 40% BG groups than in the 50% BG group (P= 0.00 and P=0.00, respectively). The pH values of the solution increased with increasing BG content. Conclusion  BG bonds are useful in the prevention of dentin demineralization. However, in this study, the 30% BG group did not affect the shortterm bond strength. The 50% BG group displayed the optimum ability to inhibit demineralization but showed the worst bond strength.

Objective  To investigate interleukin-33 (IL-33) in the arterial vascular endothelium of rabbits infected with Porphyromonas gingivalis (P. gingivalis), and to explore the relationship between P. gingivalis and atherosclerosis. Methods  A total of 24 rabbits were randomly divided into control and experimental groups. The experimental group received intravenous injection of P. gingivalis once a week for 12 weeks to establish a coronary atherosclerosis model. The rabbits in the control group were injected with equal volume of physiological saline. All the rabbits were killed after 13 weeks. The IL-33 expression levels in the arterial vascular endothelium of the rabbits were detected through immunohistochemistry, reverse transcription polymerase chain reaction, and Western blot analysis. The effects of P. gingivalis on the IL-33 expression in the arterial vascular endothelium of the rabbits were analyzed. Results  The relative expression levels of IL-33 mRNA in the vascular endothelium cells were 58.244±2.407, and the relative expression levels of IL-33 protein were 1.863±0.171 in the experimental group. The relative expression levels of IL-33 mRNA were 3.143±0.805, and the relative expression levels of IL-33 protein were 0.537±0.028 in the control group. The expression levels of IL-33 mRNA and protein of vascular endothelium cells in the experimental group were significantly higher than those of the control group (P<0.01). Conclusion  P. gingivalis infection promotes IL-33 expression levels in vascular endothelial cells and may regulate the occurrence and development of atherosclerosis.

Objective  The study aims to analyze patients with bisphosphonate-related osteonecrosis of the jaw (BRONJ). Methods  Twelve patients treated in the Department of Oral and Maxillofacial Surgery, the First Affiliated Hospital of Medical School, Zhejiang University from May 2013 to September 2015 were included. Patients’ basic situation, medication, clinical symptoms, therapies, and effects were obtained and analyzed. Results  The treatment of nine patients focused on the mandible, whereas that of three patients was on the maxilla. The clinical symptoms appeared from 10 to 80 months, with an average of about (28.00±21.42) months. Nine patients had tooth extraction history. After operation (nine patients), eight were treated, one had stable in bone exposure and three patients received conservative treatment. Conclusion  Intravenous infusion of bisphosphonates can induce BRONJ. The mandible is commonly involved and tooth extraction is a big inducement. Treatments nowadays seek to relieve clinical symptoms, but prevention is more important.

Objective  To evaluate the clinical effectiveness of tooth-alveolar bone grafts in repairing dentition defects. Methods  Forty-five patients with dentition defect were divided into two groups according to different operation methods. In group A (n=24), the tooth-alveolar bone grafts of the transplanted tooth and its surrounding alveolar bone were cut using modified surgical orthodontics and then embedded into the designated planting hole. In group B (n=21), the teeth were extracted through conventional tooth transplantation and then embedded into the prepared planting holes. The transplanted teeth were fixed for about 4–6 weeks using the orthodontic method. During the postoperation, which lasted for 2–12 weeks, root canal treatments were administered selectively. The medical effect was regularly followed up and evaluated. Results  No significant difference was found in the change of color and the periodontal ligament image between the two groups (P>0.05) whereas significant differences were observed in the degree of tooth mobility, the absorption of root and alveolar bone, and the rate of root canal treatment (P<0.05). The curative effect in group A was superior to group B. Conclusion  Tooth-alveolar bone graft transplantation has overcome some of the limitations of traditional tooth transplantation. Therefore, it is an effective means of repairing dentition defect.

Objective  This study aims to compare the changes of hyoid bone position before and after treatment of Angle class Ⅲ malocclusion using improved appliance FR Ⅲ. Methods  Forty patients with Angle class Ⅲ malocclusion were chosen and divided into two groups, namely, experimental and control. Each group had 20 patients. The young patients in the experimental group were treated using improved appliance FR Ⅲ, whereas those in the control group were treated using classic appliance FR Ⅲ. The hyoid bone position of the two groups were comparatively analyzed using an X-ray film before and after treatment. Results Compared with the condition before treatment, the condition after treatment showed that the hyoid bone position of young patients with Angle class Ⅲ malocclusion treated using improved appliance FR Ⅲ, H-FH, H-S, H-Ptm, and Ar-H-Me exhibited an increased angle (P<0.01), whereas the hyoid bone position of those treated using H-MP and H-Gn showed a decreased angle (P<0.01). The hyoid bone position of young patients with Angle class Ⅲ malocclusion treated using classic appliance FR Ⅲ, H-FH, H-S, and H-Ptm had an increased angle (P<0.05). Moreover, the hyoid bone position of those treated using Ar-H-Me had an increased angle (P<0.01), and the hyoid bone position of those treated using H-MP and H-RGn had a decreased angle (P<0.05). Conclusion  Compared with the hyoid bone position before treatment, the hyoid bone position after treatment of the young patients with Angle class Ⅲ malocclusion treated using improved appliance FRⅢ may move backward and downward, and the mandibular and hyoid bone position may move through clockwise rotation. The mandibular and hyoid bone position of young patients with Angle class Ⅲ malocclusion treated using classic appliance FR Ⅲ obtained a large angle by moving clockwise. The man-dibular bone moves backward and downward, thereby improving the hyoid bone in backward and upward directions. This condition makes a significant difference in treating the hyoid bone position of young patients with functional Angle class Ⅲmalocclusion.

Objective  This study analyzed the alveolar changes of complete unilateral cleft lip (UCCL) patients after presurgical nasoalveolar molding (PNAM) treatment using a three-dimensional method. Methods  Palate impressions of thirty UCCL patients who underwent PNAM treatment in the Affiliated Stomatology Hospital of Guangxi Medical University were taken pre-and post-PNAM. The plaster impressions were scanned using cone beam computed tomography (CBCT). The DICOM files were exported and 3D models reconstructed in Mimics 15.0. The following parameters were analyzed in SPSS 17.0: cleft alveolar gap width, alveolar arch end width, upper lip frenulum-median plane distance, alveolar shift distance, and length of alveolar section. Results  After PNAM treatment, the cleft alveolar gap width and upper lip frenulum-median plane distance decreased significantly (P<0.05). No statistically significant change was observed in the alveolar arch end width (P>0.05). Alveolar shift distances for both sides increased significantly (P<0.05), and the shift distance of major alveolar section had a more significant increase than the minor alveolar section. The lengths of the alveolar section of both sides increased significantly (P<0.05) with the length of the major alveolar section increasing more significantly than the minor alveolar section. Conclusion  The impression-CBCT method supply accurate three-dimensional data of the alveolar process. The PNAM treatment can significantly correct alveolar deformity and improve appearance.

Objective  This study investigated the influence of Hawley retainer and vacuum-formed retainer on the articulation of Chinese speech of adult orthodontic patients. Methods  A total of 20 volunteers were recruited in this randomized controlled trial in accordance with the inclusion criteria. Participants were randomly distributed into two groups, namely, the Hawley retainer group and the vacuum-formed retainer group. Speech performance was evaluated at the following time intervals: before wearing retainers (T0), immediately after wearing both upper and lower retainers (T1), 24 h later (T2), 1 week later (T3), and 1 month later (T4). The phonetic parameters were acquired through the use of computer-based voice analysis software Praat version 5.4.21, and the final data were analyzed using SPSS 19.0. Results  Both retainers can influence the phonetic parameters of vowels /e/, /i/, and /ü/ and consonants /s/, /f/, /x/, /t/, /j/, and /q/. However, the Hawley retainer showed a more remarkable effect on the articulation of Chinese speech, and the distortion of /i/, /ü/, and /s/ sounds can last for more than 1 month. Nevertheless, the recovery period of the patients’phonetic function in the vacuum-formed retainer group was within 1 month. Conclusion  Both retainers can induce distortion of some Chinese vowels and consonants in the speech of adult orthodontic patients, but the Hawley retainer can more significantly influence the articulation of Chinese speech than vacuum-formed retainer.

Objective  The study aims to investigate the correlation between total input for a period of 24 h and the daily physiological requirements to develop practical guidelines for postoperative infusion management of cleft patients. Methods Sixty-three cleft lip and palate patients under three years old who underwent surgery were included in the study. The amount of liquid taken orally as well as intravenous input were recorded for a period of 24 h and compared with the daily physiological requirements. Based on the comparison results, the amount of intravenous infusion was adjusted to match the physiological requirements. Eighty-one patients under one year old were also included to evaluate the effectiveness of the adjustment. The amount of liquid taken orally and intravenous inputs were also recorded and the correlation between total input and urine output analyzed. Results  The total input of the first group of patients significantly exceeded the physiological requirements. After adjustment, the liquid intake remained at the same level, but the total intake moved closer to the physiological requirements. Statistically significant correlations were observed between liquid taken orally and intravenous intakes, and between total intake and urine formation. Conclusion  More precise management of postoperative infusion is necessary for cleft patients. For patients less than six months old, the amount of intravenous infusion should be around 60% of the physiological requirements, whereas that for patients between six months and one year should be around 75%.

Objective  This study was conducted to investigate the effect of chewing fresh or dried betel nut on the incidence and canceration of oral mucosa disease in Haikou City in Hainan Province. Methods  Through a survey questionnaire, clinical examination, and regular follow-up, we collected clinical data from 1 722 cases and divided them into two groups, among which 704 of the afflicted people chew dried betel nut, whereas the other 1 018 chew fresh betel nut. The data were statistically analyzed using different variables which included age, number, time of onset of the disease, and the cancerous condition associated with common oral mucosa disease, including oral submucous fibrosis (OSF), oral leukoplakia (OLK), and oral Lichen planus (OLP). Results  1) The study found no significant difference in the prevalence of oral mucosa diseases between the dried betel nut group (n=704) and fresh betel nut group (n=1 018) among the 1 722 cases (P>0.05), but the peak age of oral mucosal disease was more advanced in the dried betel nut group (P<0.01). 2) The incidence within 5 years of OSF, OLK, OLP, and oral mucosa diseases in the dried betel nut group was significantly higher than that in the fresh betel nut group (P<0.01). 3) The incidence of cancer for oral mucosa disease in patients included in the dried betel nut group was significantly higher than that in the fresh betel nut group (P<0.01). Conclusion  Chewing dried betel nut is more pathogenic and carcinogenic than chewing fresh betel nut. The extremely harmful components of the dried betel nut synergistically play a vital role in the occurrence and carcinogenesis of oral mucosal diseases.

Objective  To discuss the cause of food impaction after fixed denture restoration and to analyze the therapeutic effect of gap expansion and resin repair. Methods  A total of 100 patients who suffered from food impaction after they received fixed partial denture were chosen. The cause of food impaction was analyzed. Treatment methods were applied on the basis of different causes. Gap expansion and resin repair were implemented to remove food impaction caused by the poor contact of the denture with healthy adjacent teeth. Results  The poor contact between fixed denture and healthy adjacent teeth was the major cause of food impaction (70%). The effective rate of treatment among patients who received fixed partial denture but suffered from food impaction after 3 years was 100%. Conclusion  The proposed method can be applied to treat food impaction caused by the poor contact of denture with healthy adjacent teeth.

Objective  This study aimed to construct a eukaryotic expression vector pEGFP-N1-EMP1 of epithelial membrane protein 1 (EMP1) and investigate its influence on migration and invasion of human oral tongue squamous carcinoma cells. Methods  The human EMP1 gene was amplified by reverse transcription polymerase chain reaction and then ligated into the pEGFP-N1 vector by double restriction endonuclease digestion to construct pEGFP-N1-EMP1 recombinant plasmid. After sequencing identification, pEGFP-N1-EMP1 recombinant plasmid and pEGFP-N1 plasmid were transfected into human oral tongue squamous carcinoma Tb3.1 cell line. The expression of green fluorescent protein in cells was observed after transfection using an inverted fluorescence microscope. The overexpression of EMP1 mRNA was identified at 24, 48, and 72 h after transfection by real-time fluorescence quantitative polymerase chain reaction. The effect of EMP1 overexpression on migration and invasion of Tb3.1 cells was detected by Transwell assay. Results  The full-length EMP1 gene sequence was successfully obtained. Sequence analysis showed that the EMP1 gene was inserted into the pEGFP-N1 vector correctly. Green fluorescence was observed in the transfected cells under fluorescence microscopy. The results of real-time fluorescence quantitative polymerase chain reaction indicated that the expression of EMP1 at 24 h after pEGFP-N1-EMP1 transfection was significantly higher than the other groups. Transwell assays indicated that overexpression of the EMP1 gene could significantly inhibit the migration and invasion ability of Tb3.1 cells. Conclusion  The eukaryotic expression vector of EMP1 was successfully constructed, and EMP1 overexpression was confirmed to inhibit the migration and invasion of oral tongue squamous carcinoma cells in vitro. This study laid a foundation for further investigation on the influence of the EMP1 gene on the metastasis of oral tongue squamous carcinoma and its molecular mechanism.

Objective  This study investigated the expression of BNIP3 in salivary adenoid cystic carcinoma (SACC) and its correlations to the clinicopathological features and prognosis of patients with SACC. The role of BNIP3 in the progress of hypoxia-induced autophagy was elucidated. Methods  The expression levels of BNIP3, hypoxia inducible factor (HIF)-1α, and LC3 in 65 SACC cases were detected by immunohistochemical staining method, and the correlation between the expression of BNIP3 and the clinicopathological features in SACC was analyzed. In addition, the correlations of BNIP3 gene expression with HIF-1α and LC3 gene expression were analyzed. The survival rate of patients with SACC was evaluated by univariate survival analysis. Results  BNIP3 was considerably expressed in SACC in all three histological patterns, and was positive in 41 cases (63.1%). BNIP3 gene expression was significantly correlated with histological grade (P=0.001) and HIF-1α gene expression (P=0.011). By contrast, BNIP3 gene expression was not significantly correlated with LC3 gene expression (P=0.167). The overall survival rate of patients with negative BNIP3 expression was better than that of patients with positive BNIP3 expression (P<0.05). Conclusion  BNIP3 might play a vital role in the tumorigenesis of SACC and may be a new target for gene therapy.

Objective  This study aimed to investigate the value of carbon nanoparticles as lymph node tracers in neck dissection for cN0 lingual squamous cell carcinoma patients. Methods  Ninety-six patients with cN0 lingual squamous cell carcinoma were recruited to undergo surgical treatment were randomly divided into two groups, namely, the carbon nanoparticlelabeled group (the experimental group, 50 cases) and the control group (46 cases). Carbon nanoparticle suspension was injected into the submucosal layer around the site of the primary tumor at three or four points (0.1 mL for each point) 12 h before surgery. Supraomohyoid neck dissection (SOHND, Levels Ⅰ to Ⅲ) or comprehensive neck dissection (CND, Levels ⅠtoⅤ) were performed based on the size and location of the primary tumor. All the lymph nodes were dissected and separated from the ex vivo surgical specimens for histopathological evaluation. The number, size, location, and pathological result of all the lymph nodes were compared between the two groups. Statistical analyses were conducted by SPSS 19.0 software. Results  A total of 1 137 lymph nodes were detected in 31 SOHND patients. The average number of lymph nodes detected in the experimental group was (43.79±19.23) /case, which was significantly higher than that in the control group [(30.82±8.77) /case] (P=0.019). Level Ⅲ covered the largest number of lymph nodes in the two groups. However, the number and proportion of lymph nodes found in Level Ⅱ of the experimental group were significantly higher than those of the control group (P=0.000). A total of 3 938 lymph nodes were detected in 65 CND patients. The average number of lymph nodes detected in the experimental group [(66.67±20.02) /case] was larger than that in the control group [(53.03±20.98) /case] (P=0.026). The difference in the lymph node location between the two groups was not statistically significant (P=0.354). In the two neck dissection methods, both the proportion of minute lymph nodes and the accuracy of the detected lymph nodes in the experimental group were significantly larger than those in the control groups (P=0.000). Compared with the control group, more metastases were proven by the carbon nanoparticlelabeled lymph nodes (P=0.000) in the experimental group. Conclusion  Carbon nanoparticles as lymph node tracers in patients with cN0 lingual squamous cell carcinoma undergoing neck dissection can increase the number of detected lymph nodes, especially the minute nodes. Such nanoparticles can further ensure the thoroughness of neck dissection and the accuracy of clinicopathological stage.

Objective  To explore the effects of periodontal inflammation on the functions of gut barrier (ecological barrier, mechanical barrier, and immune barrier) in mice. Methods  Twenty male C57BL/6J mice were randomly divided into periodontitis (P) or control (C) groups. The P group was subjected under a 10-day ligation with Porphyromonas gingivalis to induce periodontitis, whereas the C group was ligated with sham. Maxillae were obtained to assess alveolar bone loss. The phylogenetic structure and diversity of microbial communities in the gut were analyzed by 16s rRNA pyrosequencing. Immunohistochemical analysis was performed to determine the expressions of occludin, claudin2, and NOD2 in the ileum. Results  Compared with the C group, the P group displayed significant alveolar bone loss (P<0.001). In addition, no significant influence on the main phyla and genus Parabacteroides of the two groups was observed (P>0.05). However, the ileum of the P group showed significantly upregulated occludin, claudin2, and NOD2 (P=0.039, P=0.011, and P=0.039, respectively). Conclusion  Periodontal inflammation influences to some extent the mechanical and immune barrier functions of the mice gut.

Objective  The study seeks to investigate the expression of S100A9 and its potential role in periodontal diseases induced by diabetes. Methods  A diabetic SD rat model was established through intraperitoneal injection of streptozotocin (STZ). Hematoxylin-eosin (HE) staining was performed to study the structure of the periodontium of diabetic rats. Using immunohistochemical staining, the distribution of S100A9 expression was detected in the periodontium of diabetic rats. Expressions of Toll-like receptor 4 (TLR4) (ligands of S100A9) and p-P65/nuclear factor κB (NF-κB) were also measured. Results  The trabecular structure of alveolar bone was sparser, and lamina dura was disappeared in diabetic rats. Obviously higher expressions of S100A9 were observed in the periodontal ligament, alveolar bone, and gingival epithelial of diabetic rats than in the control rats. TLR4 expressions in the periodontal ligament, alveolar bone and gingival epithelial of the diabetic rats were also higher as compared to the control rats. p-P65 expression was not detected in the control rats, but was detected in the periodontal ligament and alveolar bone of the diabetic rats. Conclusion  Periodontium lesions in diabetes mellitus may be induced by the activation of TLR4 and NF-κB signaling pathway meditated by S100A9.

Chronic periodontitis is one of the most common oral diseases in humans, the main recognized pathogenic bacterium of which is the Porphyromonas gingivalis. Various types of viruses have been detected in periodontal disease in situ, and the joint action of viral and bacterial pathogens infection mechanism are complicated. Porphyromonas gingivalis has the characteristics resulting from the interaction with a variety of bacterium viruses, which may be the reason for chronic periodontitis being a protracted disease associated with a variety of systemic diseases. In this paper, we reviewed the relationship between Porphyromonas gingivalis and viral diseases to provide a new idea for the treatment of patients with periodontal disease and viral infections.

Recent studies found that forkhead box protein O1 (FoxO1) does not only demonstrate important biological functions in cell proliferation, gluconeogenesis, energy metabolism, and oxidative stress, but it also plays a vital role in the remodeling process of bones. FoxO1 can regulate bone mass by affecting osteoblasts, osteoclasts, and precursor cells. In this article, we review the role of FoxO1 in bone metabolism and elucidate its underlying mechanism.

In the long-term interaction between pathogens and host, the pathogens regulate the expression of related virulence genes to fit the host environment in response to the changes in the host microenvironment. Gene expression was believed to be controlled mainly at the level of transcription initiation by repressors or activators. Recent studies have revealed that small noncoding RNAs (sRNAs) are key regulators in bacterial pathogenesis. sRNA in bacteria is a noncoding RNA with length ranging from 50 to 500 nucleotides. Pathogens can sense the changes in the host environment and consequently regulate the expression of virulence genes by sRNAs. This condition promotes the ability of pathogens to survive within the host, which is beneficial to the invasion and pathogenicity of pathogens. In contrast to transcriptional factors, sRNA-mediated gene regulation makes rapid and sensitive responses to environmental cues. Many sRNAs involved in bacterial virulence and pathogenesis have been identified. These sRNAs are key components of coordinated regulation networks, playing important roles in regulating the expression of virulence genes at post-transcriptional level. This review aims to provide an overview on the molecular mechanisms and roles of sRNAs in the regulation of bacterial virulence.