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Table of Content
20 June 2004, Volume 22 Issue 03
  • Separation ofBacteroides ForsythusATCC43037 Proteins by Horizontal Two-Dimensional Gel Electrophoresis
    HUANG Ding-ming1,ZHOUXue-dong1,KUBONIWAMasae2,AMANO Atsuo2
    West China Journal of Stomatology. 2004, 22(03):  177-179. 
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    Objective To set up a rapid, efficient, reliable and accurate method for separation ofBacteriodesforsythuspro- teins.Methods BacteroidesforsythusATCC43037 cells were harvested by centrifugation, washed inTris buffer to remove excess medium, and lysed by sonication. The sonicated lysis proteins were extracted step by step with ReadyPrepTMSequential Extraction Kit (Bio-Rad). The supernatant ofB.forsythusproteins were used for two-dimensional gel electrophoresis. The first dimension IEF(isoelectric focusing) was run with Immobiline DryStrip (pH 3~10) and the second dimension SDS-PAGE was run with Ex- celgel SDS,gradient 8-18 precast gel and buffer strips. The separated proteinswere stainedwith Coomassie Brilliant Blue and silver staining kit (Plusone Silver Staining Kit, Protein, Pharmacia Biotech).Results 1. The protein spots were clear when sample cups were used in the middle of IPG strip during IEF. 2.B.forsythusproteinswere separated clearly by horizontal two-dimension- al electrophoresis and most ofB.forsythuswhole-cell proteins were acidic proteins (PI3-7).Conclusion Horizontal two-dimen- sion electrophoresis is a useful method for separatingB.forsythusproteins.

    Expression of Proliferating Cell Nuclear Antigen、bcl-2 in Gray Gingival Tissue
    CHENLin1, ZHUGuo-wei1, YANGXiao- hong1, LIUHua-qing2
    West China Journal of Stomatology. 2004, 22(03):  180-182. 
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    Objective To studythe expression of PCNA、bcl-2 protein in gray and normal gingival tissue, and to investigate the biological effect of procelain-fused-to-metals(PFM).Methods Gray gingival tissue animal model was established by PFM pros- thesis and immunohistochemistry S-P method was used to detect the PCNA、bcl-2 protein expression in gingival tissue after 3 months, 6 months in PFM groups and control group.Results The expression of PCNAhad significant difference between the 3th month group and the control group(P<0.05)|no significant difference was found between the 6th month group and the control group(P>0.05). The expression of bcl-2 had no significant difference between the 6th month group and the control group(P> 0.05).Conclusion There were a correlation between the expression of PCNA and inflammation of the gray gingival tissue;The expression of PCNA and bcl-2 protein does not indicate that gray gingival tissue had dysplasia change.

    Preparation of Lymphatic Targeting Dosage Form: Pingyangmycin Absorbed on Activated Carbon Nanoparticles
    Ming-lei1,WEN Yu-ming2, WANG Chang-mei2, LILong-jiang2, WANGXiao-yi2
    West China Journal of Stomatology. 2004, 22(03):  183-185. 
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    Objective To prepare anticancer nanoparticles for targeting therapy for oral cancer lymph node metastasis.Meth- ods The activated carbon nanoparticles(CH-NP) were prepared for drug carrier. Pingyangmycin(PYM), a high sensitive anti- cancer drug for oral squamous cell carcinoma, were selected as model drug. The activated carbon nanoparticles and PYM were mixed with saline and shaken 20 minutes so that PYMwas absorbed on activated carbon enough, resulting in a newformulation of PYM (PYM-CH-NP). The absorbency of PYMon activated carbon nanoparticles was evaluated.Results The diameter distribu- tion for CH-NP ranged form 136 nmto 540 nm, the average diameterwas 176 nm.The proportion of CH-NPto PYMwas increased and more absorbency of PYMon activated carbon nanoparticleswas achieved.Conclusion The activated carbon nanoparticles has high absorbency of PYM. The newformulation PYM-CH-NP can be used as targeting therapy of cervical lymph node metastasis by peri-cancer submucosal injection.

    Recombinant Human BMP-2 Accelerates Bone Formation of Mandibular Distraction Osteogenesis in Rabbits
    WANG Zhi-guo, HUJing, ZOUShu-juan, LIJi-Hua, GAO Zhan-wei, WANGDa-zhang
    West China Journal of Stomatology. 2004, 22(03):  186-188. 
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    Objective To study the effects of rhBMP-2 on bone formation of mandibular distraction osteogenesis in rabbits. Methods Bilateral mandibular osteotomieswere performed in 12 mature rabbits. 5 mg rhBMP-2 withthe collagen carrierwas im- planted in the osteotomy site of one side of the mandibles. Only the collagen sponge was placed in the contra-lateral side as con- trol. The mandibles of 8 rabbitswere lengthened by 6mm using a custom-made distractor. At 4 weeks after the end of distraction, all animalswere killed and the distracted calluseswere harvested and processed for histological, scanning electron microscopic, as well as Ca/P ratio analysis.Results The regenerated bone was found in the distraction gap after mandibular lengthening. The mandibular side treated with rhBMP-2 had greater amounts of newbone formation and earliermineralization than contra-lateral side (non-rhBMP-2 treated).Conclusion Recombinant human BMP-2 appears to be able to accelerate bone formation of mandibular distraction osteogenesis in rabbits.

    Effect of Fas Gene Transfection on Tumourigenicity and Proliferation of Transplanted Tumor of Oral Squamous Cell Car- cinoma Cell Line Tca8113
    HOUJin-song1, HUANGHong-zhang2, WANG Jian-guang2,PAN Chao-bin2
    West China Journal of Stomatology. 2004, 22(03):  189-191. 
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    Objective To investigate the effect of fas gene transfection and monoclonal anti-fas antibody on tumorigenicity and proliferation of transplanted tumor of Tca8113 cell.Methods Plasmid including fas gene was transfected into Tca8113 cell by lipofectamine kit. Some transfected cells were treated by monoclonal anti-fas antibody after 48 hours since transfection. Untrans- fected cell (control), fas-tansfected cell and fas-transfected cell treated with antibody were transplanted to nude mice subcuta- neously. Growth of transplanted tumorwas observed and recorded regularly. Animals were sacrificed and tumor samples were har- vested at the end of experiment. Fas expression in each neoplasm was assessed by RT-PCR. Apoptosis, proliferation and expres- sion of fas protein in tumor tissue were measured by flow cytometry (FCM).Results Tumor occurred much later in fas-transfect- ed group and fas-transfected plus antibody treated group. Growth arrest was found in them. RT-PCR and FCM suggested that fas- transfection up-regulated the expression of fas mRNA and protein, increased apoptosis index (AI). But no effect on proliferation index (PI) was observed. Monoclonal anti-fas antibody did not effect the expression of fas mRNA and protein, but increased AI and decreased PI.Conclusion Fas-transfection suppressing tumorigenesis of Tca8113 cell transplanted in nude mice might be caused by up-regulation of expression of fas gene and enhancement of apoptosis. However, anti-fas antibody suppressing tumorige- nesis might be associated with activation of apoptosis and repression of proliferation.

    FEM Study on Displacement, Position of Rotation Center and Stress Distribution of PDL under Various Loading Force Systems
    LIUDong-xu1, WANG Chun-ling1, FUChuan-yun1, ZHANGXiao-yan1, ZHENGXiao-zhong2
    West China Journal of Stomatology. 2004, 22(03):  192-195. 
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    Objective To investigate the displacement, the position of rotation center and the stress distribution of PDL under different loading force system (Moment/Force ,M/F) by simulating clinical loading force system.Methods Athree-dimensional finite element model of upper central incisor, which consists of 945 isoparametric elements and 1 245 nodes was developed. The displacement, the position of the rotation center and the stress distribution of PDL were analyzed under 13 types of loading force system.Results 1.Different force system led to different types of tooth movement. When M/F= -9.15∶1, -10.30~-10.50∶1 and -10.90∶1, it brought the result of controlled tippingmovement, the bodilymovement and the rootmovement|2. The graph of the center rotationwas a hyperbolic asymptotic line:Mx/Fy= -10.50 (horizontal axis) and L=6.75(vertical axis). Moreover, a little change of M/F between -9.15 and -10.90 led to apparent change of the position of rotation center. 3. The maximum strain and stress during the tipping movement were 1.47×10-2MPa and -2.81×10-2MPa, and during the bodily movement the results were 1.10×10-2MPa and -1.86×10-2MPa, while during the root movement were 0.96×10-2MPa and -2.58×10-2MPa.Con- clusion 1. Different force system (M/F) leads to differenttypes of toothmovement. 2. It is necessaryto adjustthe force system accurately to obtain prescient tooth movement, especiallywhenM/F changes between-9.15∶1 and-10.90∶1. 3. This study sug- gested that the tooth movement style and the force system (M/F) should be controlled to protect the periodontal tissue.

    The Effect of Left Bacteria in the Root Canal on Prognosis of the Root Canal Therapy
    HEJin-mei, BIANZhuan, FAN Ming-wen, FANBing
    West China Journal of Stomatology. 2004, 22(03):  198-200. 
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    Objective To study the effect of the left bacteria on the root canal therapy.Methods 50 single-rooted teethwith chronic apical periodontitis were divided into two groups, one was instrumented with step-back technique and 2.5%NaOCl ultra- sonic irrigation for 3 min, then filled with Thermafil.Samples were taken after instrumentation to culture. The other was treated with traditional RCT at three visits.Results In 24 months the apical radiolucency were greatly reduced in all cases. There weren′t significant relationship among the postoperative pain and the left bacteria, the degree of the obturation or the pre-operative symptoms (P>0.05).Conclusion The effect of left bacteria in root canal filled with Thermafil wasn′t observed.

    Compositive Treatment of Square Face
    XIA De-lin, GUI Lai, ZHANG Zhi-yong, JIN Ji, NIU Feng, LUO Jin-chao
    West China Journal of Stomatology. 2004, 22(03):  201-203. 
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    Objective To investigate the treatment of square face with compositive methods.Methods Atotal of 71 patients with square-shape face were treated. According to the different face skeleton and desire of patient, mandible angle curved-osteoto- my and mentoplastywere used as main methods and zygomatoplasty, buccal fat pad resecting and other aesthetic methods as assis- tant methods to recontour the whole face skeleton.Results The face skeleton of all patients was improved with satisfaction. The following-up period was 6 months to 2 years. In this patients group,massive haemorrhage was occurred during operation in one pa- tient, mental nerve of one side was injured in two patients, the lip mucosa was wounded in five patients.Conclusion Mandible angle curve-osteotomy and mentoplasty combined with other assistant aesthetic operations were ideal methods to recontour square face. The result was satisfactory.

    A Retrospective Study on 615 Cases of Minor Salivary Gland Tumors
    LI Yi-ning, HE Zhi-xiu, LIULai-kui,HEHua- wei
    West China Journal of Stomatology. 2004, 22(03):  204-206. 
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    Objective To analyse the clinical and pathological characterstics ofminor salivary gland tumors.Methods Aret- rospective analysis on 615 cases of intraoral minor salivary gland tumors from 1990~2002 with a confirmed pathologic diagnosis was carried out.Results In 615 cases ofminor salivary gland tumors, 265 caseswere benign, 350 casesmalignant. Pleomorphic adenoma was the most common entity and accounted for 81·1%of all benign tumors. Adenoid cystic carcinoma comprised 32·9% of the malignant sample and was the most frequent malignant tumor. The principal location was the palate. Female was a little more of the benign and a male prevalence was observed on the malignant tumor. The mean age of patients with benign and malig- nant tumors were 40·9 and 49·1 years old, respectively (P<0·05).Conclusion We consider extraordinary manifestation as histopathology, tumor, primary location, age and sex, presentingthe results of a reviewof our experiencewiththose minor salivary gland tumors.

    Application of Facial Canal Dissection for Recovery of Facial Nerve after Operation of Parotid Carcinoma
    HANSi-yuan, WANG Yu-xin,SONG Tao
    West China Journal of Stomatology. 2004, 22(03):  207-210. 
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    Objective To studythe recoverymethod of facial nerve function and to compare the clinical effect after operation of parotid carcinoma, which invades stylomastoid foramen and peripheral bone in deep lobe.Methods Three operation methods were taken:①The tumor, parotid, invasive facial nerve and bone around the tumor were resected with transplatation of facial nerve.②Tumor parotid and facial nerve were resected without transplatation of facial nerve.③Tumor and parotid were dislocated from facial nerve,keeping the continuity of facial nerve.Results For the first method, facial nerve function of 68·2% patients came back to the patientswithout facial paralysis before operation, while facial nerve function of 16·7% patients came back to the patients with facial paralysis before operation. There was obvious difference between them(P<0·05).To the patients with facial paralysis before operation,the first and the second method were taken.The ratio of local control was 33·3% and 10·0% respec- tively.And survival for 5 years were 25·0% and 10·0% respectively. There was no obvious difference between the two methods (P>0·05). To the patients without facial paralysis before operation, three methods of operation were taken.The ratio of local control was 77·3%, 48·0% and 33·3% respectively. And survival for 5 years were 86·4%, 52·0% and 41·7% respectively. There was obvious difference between the firstmethod and the othertwo (P<0·05). There was no obvious difference between the second and the third methods (P>0·05).Conclusion Facial canal dissection in operation of parotid carcinomawith recovery of facial nerve can not only resect tumor completely, but also fit for development of functional surgery. It is an ideal method for surgery operation.

    Treatment of Bell′s Palsy with Combination of Traditional Chinese Medicine and Western Medicine
    WANGXiao-hong1, ZHANGLi-ming1, HANMei2, ZHANGKe-qing1, JIANG Jiao-Jiao1
    West China Journal of Stomatology. 2004, 22(03):  211-213. 
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    Objective To evaluation the clinical effect of combination of traditional Chinese medicine and western medicine on Bell′s palsy.Methods 83 patientswith Bell′s palsywere randomly divided intotwo groups(trail group 54 cases and control group 29 cases). Patients in two groups were treated with medicine, acupuncture, physiotherapy, while patients in the trail group were treated with massage and functional exercise as the same time. The results of both groups were evaluated according to Portmann′s Simple Scale.Results The score before treatment of trail group was 2.907±1.794, while control group was 2.931±2.034. And the score after treatment of trail groupwas 18.593±1.743, while control groupwas 9.862±3.091. Score of the function of facial muscles obtained from trail group was distinctly higher than thatwas from the control group (P<0.01), as well as the im- provement index (P<0.01, trail group: 0.844±0.095, control group: 0.712±0.129).Conclusion There is significant cu- rative effect and suitability in the treatment of Bell′s palsywith combination of traditional Chinese medicine and western medicine. The improvement of facial muscles′motive function pre- and post-treatment and quantitative evaluation of curative effect can be ob- jectively obtained by evaluation of facial muscles′function.

    Clinical Study on the Effect of Vitapex Paste in Apexification
    WENG Qiao-feng
    West China Journal of Stomatology. 2004, 22(03):  214-215. 
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    Objective The purpose of this article was to observe the effect of Vitapex Paste in apexification.Methods 64 younger permament teeth with underdeveloped apex and necrotic pulpwere randomly selected. After root canalswere prepared and sterilized, Vitapex paste was used in the apexification. All the teeth were observed for three years.Results 24 teeth(37.5%) were successful, 37 teeth (57.81%) were progressive, 3 teeth (4.69%) were failed. Altogether 61 teethwere effective, the ef- fective rate was 95.31%.Conclusion Vitapes paste was effective for the younger permanent teeth in the apexification.

    Research of Cranio-occlusional Change of Skeletal ClassⅢMalocclusion in Permanent Dentition Treated by the Multiloop Edgewise Arch Wire Technique
    JINChang-wei, LINJiu-xiang, XUBao-hua
    West China Journal of Stomatology. 2004, 22(03):  216-219. 
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    Objective To analyze the mechanics in correction of skeletal classⅢmalocclusion with Multiloop Edgewise Arch Wire(MEAW).Methods 15 patients with skeletal classⅢmalocclusion were treated with MEAW technique. Cephalometric analysis was performed with pre-treatment and post-treatment cephalograms. Pairedt-test was conducted to assess the treatment effects.Results L6-XI decreased by 2·87 mm, L6/MP increased by 8·60°, L1-XI decreased by 2·60 mm, OP/MP increased by 2·33°.Skeleton changed a little. There was no significant change in the soft tissue.Conclusion ①Dento-alveolar compensa- tion is the main change after the treatment byMEAWtechnique;②The improvement in molar relationship and overjet is achieved with upright and distal movement of the lower posterior teeth;③The lower anterior teeth moved lingually and protracted. Occlusal plane is flattened.
    Clinic Significance of Morphometric Study on Squamous Cell Carcinoma of Floor of Mouth
    ZHAOHong-wei1, LILong- jiang1,XIE Wen-yang2
    West China Journal of Stomatology. 2004, 22(03):  220-223. 
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    Objective The purpose of this study was to investigate the role of cell morphometric factor (FC) and the average density of light in squamous cell carcinomas at the floor of mouth by immunohistochemical method (SABC).Methods The slices made from the paraffin specimens of 28 patients with squamous cell carcinomas at the floor of mouthwere stained by the immuno- histochemical technique withmonoclonal antibody CK10and CK14.In these stained slices the FC parameters and the average density of light were measured by using an image analysis system.Results In primitive tumors, the FC parameters and the average den- sity of light were significantly different between positive and negative lymph nodes metastasis or between different pathological grades(P<0.05 or <0.001).The significancewas not existed among clinical stages(P>0.05). The difference of FC expressed in positive and negative lymph nodesmetastasiswere significant(P<0.05 or <0.001).Conclusion The cell morphometric fac- tor (FC) parameters and the average density of light of CK10and CK14could be used as a biological guideline to evaluate the clini- cally infiltrative and metastasic potential of squamous cell carcinoma at the floor of mouth.

    The Computer-aided Design and Manufacture of Unilateral Orbital Defect Restoration
    WUGuo-feng, ZHAO Yi-min, LIU Xiao-fang, LIDi-cheng, QULe, LIULu
    West China Journal of Stomatology. 2004, 22(03):  224-226. 
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    Objective By reverse engineering and rapid prototyping techniques to found a new design method of maxillofacial restoration.Methods By laser scanning apparatus the plaster face model was scanned and the primitive face point data were ac- quired. With the reverse engineering software, the point data were reconstructed to one smooth face surface image and the defect orbital tissue shape data was obtained from the normal contralateral tissue data in the software. The model designed the three-di- mensional data of defect part and the rapid prototyping technique made the resin orbital restoration.Results The laser scanning apparatus acquired the distinct and precise model data of the plaster face-model. The Digisurface retrograde engineer software suc- ceeded to fulfill the unilateral orbital defect computer-aided design. The orbital restoration inosculated the plastermodel tightly and symmetrically.Conclusion The reverse engineering software and rapid prototyping technique could finish the computer-aided de- sign and manufacture of the unilateral orbital defect restoration smoothly and satisfactorily.

    Restoring Ceramo-metal Tooth by Using Self-made Crown Post-core in order to Re-utilize the Broken Crown of the Base Tooth
    QUANMiao
    West China Journal of Stomatology. 2004, 22(03):  227-228. 
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    Objective To explore a newmethod and its clinical significance of restorative ceramo-metal by using a self-made crown post-core to reutilize the broken crown.Methods 18 teethwere restoredwith ceramo-metal tooth by using self-made crown post-core in order to preserve the old broken base tooth. The patients′own feelings and clinical effect were observed.Results  Followed up for 1~3 years, 83.3% (15 teeth) of the patients were successful. Among the group one was failed because of bad condition of post and caused split of tooth root. The second failurewas caused by uneven force by coherent paste uneven. The third failure was caused by tight biting force and caused the tooth broken.Conclusion Restoring ceramo-metal tooth by using self- made crown post-core in order to re-utilize the broken crown of the base tooth is convenient and low cost and is suitable for basic hospitals.

    In vitroCulture and Biological Characteristics of Cranial Neural Crest Stem Cell
    TIANWei-dong,JIANGHong-bing, LIU Lei, TANG Wei, LI Xiao-dong
    West China Journal of Stomatology. 2004, 22(03):  229-231. 
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    Objective According to development biology, cranial neural crest stem cell (CNCSC) can differentiate into pre- cursor cells of tooth, jaw and peripheral nerve system, butin vitrostudy is less reported. In the present study, CNCSCwere dis- sociated and culturedin vitro,and the biological characteristics of CNCSCwas investigated.Methods Cranial neural tubes, dis- sected from mouse E9d, were explanted onto fibronectin-coated dishes. CNCSCs emigrated from the explanted neural tubes, cul- tured in a serum-free medium containing modified DMEM/F12.Biological characteristics of CNCSC were detected by morphology, nuclear labeled with BurdU and immunocytochemistry.Results Fibroblast-like CNCSCs demonstrated the characteristics of stem cell, such as clonality, self-renewal and multipotentiality. The result of immunocytochemical stain showed that CNCSC expressed HNK-1 antigen.Conclusion CNCSC were cultured successfully, providing a experimental basis for study on tooth/jaw-like dif- ferentiation of CNCSCin vitro, especially providing a cell source for investigating tooth/jaw regeneration.

    Establishment of a Culture System of Chick Embryo for Mouse Tooth Germ Development
    YAN Zheng-bin,TIAN Wei- dong, LIULei, HOUJin-qiu,CHENXi-zhe,LI Zhi-yong
    West China Journal of Stomatology. 2004, 22(03):  232-234. 
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    Objective To establish a new culture systerm for mouse tooth germs in chick embryo.Methods The mandibular first molar germ fragments of 15 embryonic days′Kunming mouse embryo were implanted into the lateral mesenchyme of 4~5 days′chick embryo wing buds in ove. Eggs were reincubated and implanted tissues were examined by histochemistry.Results  The cultured tooth germ development continued from cap stage to latest bell stage. The ameloblast and the odontoblast all differen- tiated maturely and secreted matrix.Conclusion 4~5 days′wing buds chick embryo could serve as developing the mouse tooth germs and demonstrate well physiological process of differentiation and morphogenesis.

    Investigation of the Expression of Calcitonin Receptor mRNA in Human Osteoclasts on Deciduous Teeth
    CHENMing1, LIANGXing2, BAOXiang-jun2, WANGHang2, SUNHui-qiang2,LUShao-heng2
    West China Journal of Stomatology. 2004, 22(03):  235-237. 
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    Objective To investigate the expression of calcitonin receptor mRNA in the osteoclasts of the resorbing deciduous teeth.Methods After fixing the collected deciduous teeth, toluidine blue was performed and tartrate-resistant acid phosphatase (TRAP) staining was used to identify the osteoclasts on the resorbing surface of human deciduous teeth and in situ hybridization of calcitonin receptor mRNA to show its existence.Results There were a number of TRAP positive osteoclasts on the root surface which showed the expression of calcitonin receptor mRNA.Conclusion On the resorbing surface of human deciduous teeth there are osteoclasts that express calcitonin receptormRNA, so it is feasible to use this kind of osteoclasttotestthe effect of external fac- tors on the expression of CTR mRNA.

    Centrosome Hyperamplification in Oral Precancerous Lesions and Squamous Cell Carcinomas
    CAI Yang1, LI Bing-qi2, CHENG Qian-ming2
    West China Journal of Stomatology. 2004, 22(03):  238-241. 
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    Objective It is currently considered that the defect of mitotic spindle caused by centrosome abnormalities may be one of the reasons for the development of aneuploidy in tumors. This study attempted to elucidate the possible role of centrosome defects in the development and progression of OSCC by investigating the frequency of centrosome amplification in oral precancerous lesions and OSCC.Methods Formalin-fixed, paraffin-embedded tissues of 12 cases of normal oral epithelium, 22 case of dyspla- sia with different degree epithelium dysplasia and 32 cases of OSCC with different differentiation were investigated for centrosome status by using indirect Immunofluorescence double staining with antibodies to centrosome protein gamma-tubulin and cytokeratin. The differences and the change trend of centrosome status in these groupswere statistical analyzed by SPSS10·0.Results Normal oral epithelium showed normal centrosomes in epithelium cells, while 16 of 22 cases (72·73%) of dysplasia(DYS) and 27 of 32 cases (84·38%) of OSCC showed the evidence of centrosome amplification and morphological abnormalities characterized by huge size, clump or supernumerary centrosomes in a fraction of epithelium ortumor cells. The percentage of cellswith abnormal centro- somes increased gradually from mild-dysplasia epithelium to poorly differentiated OSCC, which positively correlated with the histo- logicalcytologic grade of oral precancerous lesions and OSCC(P<0·01).Conclusion Centrosome amplification was an early event and thatmight play a role in the establishment and perhaps the progression of OSCC. There might be some direct relationship between centrosome defects and the cellularmorphological phenotype characteristics of dysplasia and OSCC. Centrosome amplifica- tion could be served as an alternative diagnostic indicator of dysplasia and the intervention of centrosome cycle might serve as a particular way for the prevention and treatment of OSCC in the future.

    The Morphological Study of Bone-implant Interfacesin vivo
    DING Zhong-juan1, DONG Qiang1, XIAO Xu-hui1, DONG Bao-cai1, WANG Jing1,LIANG Xing2
    West China Journal of Stomatology. 2004, 22(03):  242-245. 
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    Objective To evaluate the bone-implant interfaces of two kinds of implants with different surfaces in different time in vivo.Methods CDIC and ITI-TPS solid-screw cylinder pure titanium implants were selected and implanted in the regions of posterior molars of rhesusmonkeys. 1 month, 2 months, 3 months and 1 year after surgery, the bone-implant interfaceswere eval- uated respectively through oral examination, X-ray inspection, lightmicroscope and scanning electron microscope (SEM) observa- tion.Results None of the implants was loose. Soft tissue around implants appeared no inflammation. There were no apparent transparent shadow around the implants interfaces in X-ray photos except little angle-shaped absorption was showed in neck region of CDIC implants of one-month. New bone was observed around implants of one-month through light microscope and SEM. More bone growing around ITI implants were seen than that around CDIC implants except the interfaces of one-year.Conclusion The osseointegration of ITI implants are better than that of CDIC implants during three months after implanting without loading, The bone formation at the interfaces of ITI and CDIC implants has no significant difference after one year without loading.

    The Effect of Intraperitoneal Heparin on Distribution of ACC-M in Lung and Liver
    WANG Xin-jun1, CHEN Wan-tao2, ZHOUZheng-yan1, MAYan-qing3,ZHOUXiao-jian2
    West China Journal of Stomatology. 2004, 22(03):  246-247. 
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    Objective To investigate the effect of intraperitoneal heparin on the adhesion of highly lungmetastasic adenoid cys- tic carcinoma cell line (ACC-M) in lung.Methods 3HTdR labeled ACC-M cells were injected by intravenous infusion after in- traperitoneal injection with 200 units heparin. 4 mice of each group were killed at 2 h, 6 h and 18 h after infusion. The relative radioactivity (CPM) in lung and liverwas detected.Results 3H-activity pergram in lung of heparin groupwas lowerthan control at the same time. There was significant difference between the two groups (P<0.001). There was no difference between the two groups in liver (P>0·05).Conclusion The results of this study suggest that the highly lungmetastasis characteristic of ACC-M may be partially inhabited by the use of intraperitoneal heparin.

    Experimental Study of Periosteal Osteoblasts Adhesion to Artificial Bone Scaffolds Based on Rapid Prototype
    XIANSu- qin1,CHAI Feng2, ZHAO Yi-min2, WANG Zhong-yi2,LIUXiao-fang2, LITiao-cheng3
    West China Journal of Stomatology. 2004, 22(03):  248-251. 
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    Objective To study the biocompatibility of bone engineering scaffolds designed and fabricated by CAD and Rapid Prototyping techniques.Methods Infant rat calvarias osteoblastswere isolated and expandedin vitroand the cells (2nd passage) were seeded onto scaffolds with porosity 80%,90%,95% at a density of 2·06×109/L. Cell adhesion number and morphology were measured with SEM after 4 days, 10 days co-culture.Results ①The osteoblasts′adhesion amounts increased with culture time in three porosity group (P<0·05), but the increase were different among three groups, 80% group was 0·35×105, 90% group was 2·84×105;②Through SEM observations,it showed that osteoblasts adhered to all scaffolds well.Conclusion The scaffolds designed and fabricated by CAD and rapid prototyping own a good cellular biocompatibility. The results suggest the feasi- bility of using such scaffold fabricating method for bone tissue engineering research and clinical therapy.

    The Effect of Cryotreat on Tensile Properties of Medium Melting-point and High Melting-point Castable Alloy
    CHEN Meng-shi1, ZHUZhi-min2, LI Ning3
    West China Journal of Stomatology. 2004, 22(03):  252-254. 
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    Objective To introduce cryotreat technique into prosthetic dentistry by testing the tensile properties of CW-H Co- Cr-Mo cast alloy before and after cryotreat and to observe the image changes by SEMto study the mechanismthat changes the ten- sile properties of the alloy.Methods 15 CW-H cast alloy were divided into 3 groups, i.e. control group (G1), cryotreated group (G2) and cryotreated plus post-cryogenic treated group (G3). The gauge test technique was employed to test the modulus of elasticity. Then the strength and percentage elongation (PE) were tested. SEM images were used to analyze the mechanism that improved the tension properties of the alloy.Results For CW-H alloy the strength and the modulus of elasticity of both G2 and G3 were effectively increased but PE effectively decreased than G1. There was no effectively difference between G2 and G3, but G3 was largerthan G2. SEMimages of G2 and G3 showed that secondary-carbonide separated out all overthe alloy.Conclusion  The results obtained above suggest that cryotreat is an effective method in enhancing tensile properties of CW-HCo-Cr-Mo cast al- loy.

    Designing Dental Manpower Index to Evaluate Dental Manpower Resources
    LI Gang, NI Zong-zan
    West China Journal of Stomatology. 2004, 22(03):  255-258. 
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    Objective The purpose of this investigation is to find out a method to evaluate dental manpower resources.Meth- ods We selected population, GDP, number of dentist and number of different oral health professionals from certain internet sta- tions, published books and journals from 1996 to 2000 as our investigating data.Results Data was collected from 100 countries. Our investigation found that the design of dental manpower index to evaluate dental manpower resources was effective and conve- nient.Conclusion Dental manpower index is a good method to evaluate dental manpower resources.