West China Journal of Stomatology ›› 2018, Vol. 36 ›› Issue (6): 602-608.doi: 10.7518/hxkq.2018.06.004

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Effects of calcium ion on the migration and osteogenic differentiation of human osteoblasts

Qun Lei,Dong Lin,Wenxiu Huang,Dong Wu,Jiang Chen()   

  1. Stomatological Hospital of Fujian Medical University, Fuzhou 350000, China
  • Received:2018-01-12 Revised:2018-09-23 Online:2018-12-01 Published:2018-12-12
  • Contact: Jiang Chen E-mail:dentistjiangchen@163.com
  • Supported by:
    National Science and Technology Support Program(2012BAI17B03-6);Youth Found of Fujian Provincial Health and Family Planning Commission(2015-1-66);Doctoral Research Fund from Stomatological Hospital of Fujian Medical University(2015-KQ-YY-BS-6)

Abstract:

Objective This study aimed to investigate the effect of calcium ion (Ca 2+) on the migration and osteogenic differentiation of human osteoblasts and explore the proper concentration and correlation mechanism. Methods A series of Ca 2+solutions with different concentrations was prepared. Osteoblast migration was assessed by Transwell assay, and proli-feration was studied via the CCK-8 colorimetric assay. The mRNA expression of osteogenic genes was examined via reverse transcription-polymerase chain reaction (RT-PCR), and the mineralized nodule was examined by alizarin red-S method. After calcium sensitive receptor (CaSR) antagonism, Ca 2+-induced migration and osteogenic differentiation were analyzed. Results In the migration experiment, 2, 4, and 6 mmol·L -1Ca 2+could promoted osteoblast migration at three timepoints (8, 16, and 24 h), whereas 10 mmol·L -1 Ca 2+considerably inhibited migration at 8 h. The Ca 2+ concentration range of 2-10 mmol·L -1 could promote proliferation, osteogenic differentiation, and mineralization of human osteoblasts. Moreover, mineralization was predominantly induced by 8 and 10 mmol·L -1Ca 2+. CaSR antagonism could reduce Ca 2+-induced migration and osteogenic differentiation of human osteoblasts. Conclusion Low Ca 2+ concentration favored osteoblast migration, whereas high Ca 2+ concentration favored osteogenic differentiation. The Ca 2+ concentrations of 4 and 6 mmol·L -1 could substantially induce osteo-blast migration and osteogenic differentiation, and the Ca 2+-CaSR pathway participated in signal transduction.

Key words: calcium ion, human osteoblast, migration, calcium sensitive receptor, bone substitute

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