Stromal cell-derived factor-1 inducing metastasis squamous cell carcinoma of head and neck induced via integrin αvβ3-CXC chemokine receptor 4/7 biological axis

doi:10.7518/hxkq.2018.04.010

Abstract

Abstract:

Objective To observe the influence of LM609/AMD3100/CCX754 on chemotactic capability, cytoskeleton, and expression of integrin αvβ3 protein of squamous cell carcinoma of head and neck (SCCHN) cell line PCI-13 induced by stromal cell-derived factor-1 (SDF-1) in vitro. Methods Migration assays, flow cytometry and immunofluorescence were used to observe the effects of SDF-1, LM609, AMD3100 and CCX754 on the migration, cytoskeleton and the expression of integrin αvβ3 protein in PCI-13 cell lines. Results SDF-1 favored PCI-13 cell migration, pseudopod formation, and activities of integrin αvβ3 phosphorylation. LM609, AMD3100, and CCX754 blocked all these effects. Conclusion SDF-1 can induce metastatic SCCHN by integrin αvβ3-CXC chemokine receptor (CXCR) 4/CXCR7 axi. LM609, AMD3100, and CCX754 and can reduce the regulation of SDF-1 on SCCHN activity.

Key words: stromal cell-derived factor-1, CXC chemokine receptor, squamous cell carcinoma of head and neck

CLC Number:

R739.8

Peng Li, Wenlu Li, Jinxing Qi. Stromal cell-derived factor-1 inducing metastasis squamous cell carcinoma of head and neck induced via integrin αvβ3-CXC chemokine receptor 4/7 biological axis[J]. West China Journal of Stomatology, 2018, 36(4): 398-403.

Figures/Tables 4

References 18

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