Effects of paeonol on the function of bone marrow-derived macrophage from Porphyromonas gingivalis-induced mice

doi:10.7518/hxkq.2017.02.006

Abstract

Abstract:

Objective This work aims to examine the effects of paeonol treatment on the ability of bone marrow-derived macrophage (BMM) to excrete inflammatory factors and to differentiate into osteoclasts upon induction with Porphyromonas gingivalis (P. gingivalis). This work also aims to investigate the underlying mechanisms of these abilities. Methods BMM culture was treated with different paeonol concentrations at for 1 h and then stimulated with P. gingivalis for 24 h before programmed death-ligand 1 (PD-L1) was quantified with flow cytometry. Tumor necrosis factor-α (TNF-α), interleukin (IL)-1β, and IL-6 were detected by enzyme-linked immunosor-bent assay (ELISA). The BMM culture was treated with the receptor activator for nuclear factor-κB ligand (RANKL) and macrophage colony-stimulating factor (M-CSF), and then with paeonol for 1 h prior to induction with P. gingivalis. Then, osteoclast formation was assessed using tartrate resis-tant acid phosphatase (TRAP) staining. The osteoclast-related proteins TRAP and receptor activator of nuclear factor-κB (RANK) were quantified by Western blotting. Results Paeonol was nontoxic to BMM within a range of 10-50 μmol·L^-1. Flow cytometry showed that paeonol inhibited PD-L1 expression in P. gingivalis-induced BMM in a dose-dependent manner. ELISA indicated that paeonol dose-dependently inhibited the excretion of TNF-α, IL-1β, and IL-6 by P. gingivalis-induced BMM (P<0.01). TRAP staining revealed that paenol treatment inhibited the differentiation of P. gingivalis-induced BMM into osteoclasts. Western blot results suggested that paeonol decreased the expression of TRAP and RANK in BMM. Conclusion Paeonol dose-dependently inhibited the excretion of the inflammatory factors TNF-α, IL-1β, and IL-6 by P. gingivalis-induced BMM in a dose-dependent manner. Moreover, paenol treatment prevented the differentiation of P. gingivalis-induced BMM differentiation into osteoclasts.

Key words: paeonol, Porphyromonas gingivalis, bone marrow-derived macrophage, osteoclast, inflammatory, differentiation

CLC Number:

R781.4

Zhu Chen, Lingkai Su. Effects of paeonol on the function of bone marrow-derived macrophage from Porphyromonas gingivalis-induced mice[J]. West China Journal of Stomatology, 2017, 35(2): 139-144.

Figures/Tables 6

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组别	IL-1β	IL-6	TNF-α
对照组	41.86±10.010	556.7±27.850	27.03±13.050
10 μmol·L^-1丹皮酚对照组	36.51±9.590	526.0±2.960	16.88±6.095
30 μmol·L^-1丹皮酚对照组	54.41±8.326	481.2±4.462	6.55±0.608
50 μmol·L^-1丹皮酚对照组	189.80±56.230	447.2±3.256	14.93±1.994
阳性刺激组	906.20±22.930	7 172.0±25.000	1 319.00±27.370
牙龈卟啉单胞菌+10 μmol·L^-1丹皮酚组	34.48±26.310^***	4 977.0±72.160^**	621.10±85.850^***
牙龈卟啉单胞菌+30 μmol·L^-1丹皮酚组	48.09±12.380^***	2 637.0±138.200^***	267.10±10.670^***
牙龈卟啉单胞菌+50 μmol·L^-1丹皮酚组	53.61±3.067^***	872.8±26.610^***	277.90±53.680^***