West China Journal of Stomatology ›› 2016, Vol. 34 ›› Issue (3): 291-294.doi: 10.7518/hxkq.2016.03.015

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Effects of pulsed ultrasound and pulsed electromagnetic field on the extracellular matrix secretion of rat bone marrow mesenchymal stem cell pellets in chondrogenesis

Zhang Zhi1, Tang Na2, Wang Jue1, Zhao Zhihe1, Tan Lijun1   

  1. 1. State Key Laboratory of Oral Diseases, Dept. of Orthodontics, West China Hospital of Stomatology, Sichuan University, Chengdu 610041, China; 2. Dept. of Stomatology, People’s Hospital, Sichuan Province, Chengdu 610072, China) Supported by: National Natural Science Foundation of China (30900287, 81030034, 30900286). Correspondence: Tan Lijun, E-mail: orthotan@vip.163.com.
  • Received:2015-12-16 Revised:2016-02-01 Online:2016-06-01 Published:2016-06-01

Abstract: Objective To study the effects of pulsed ultrasound (PUS) and pulsed electromagnetic fields (PEMF) on the secretion of extracellular matrix from a culture complex during in vitro chondrogenesis.Methods All the rat bone marrow mesenchymal stem cell pellets were cultured in achondrogenic medium. Different intensities of PUS (100, 150, and 200 mW·cm-2) and PEMF (1, 2, and 5 mT) were applied to the cell pellets for 2 weeks. Group N was cultured without PUS and PEMF stimulation as control. The culture medium was collected after 2 weeks of culture. Enzyme-linked immunosorbent assay (ELISA) was used to detect the type of collagen and glycosaminoglycan (GAG) in the culture medium. Results PUS increased the secreting-type collagen and GAG from cell pellets compared with group N (P<0.05), whereas there was no difference in different intensities (P>0.05). PEMF had no significant effect on the secretion of the type of collagen (P>0.05). A PEMF of 1 mT had no significant effect on the secretion of GAG (P>0.05). A PEMF 2 and 5 mT decreased the secretion of GAG (P<0.05). Conclusion To prevent the secretary of extracellular matrix may play a role in chondrogenic effect of PEMF.

Key words: pulsed ultrasound, pulsed electromagnetic field, chondrogenesis, extracellular matrix

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