West China Journal of Stomatology ›› 2012, Vol. 30 ›› Issue (4): 425-429.doi: 10.3969/j.issn.1000-1182.2012.04.024

Previous Articles     Next Articles

The effect of bone morphogenetic protein-2 and osteoprotegerin in trans-sutural distraction osteogenesis

Yao Yusheng1, Huang Hua1, Chang Shimin2, Wang Chengyue1, Wang Guijun3.   

  1. 1. Dept. of Oral and Maxillofacial Surgery, The Second Affiliated Hospital of Liaoning Medical College, Jinzhou 121000, China; 2. Dept. of Oral and Maxillofacial
    Surgery, Beijing Friendship Hospital, Beijing 100050, China; 3. Pharmacy Department, Liaoning Medical College, Jinzhou 121000, China
  • Received:2011-03-14 Revised:2011-11-05 Online:2012-08-01 Published:2012-08-01

Abstract:

Objective To determine if locally administered bone morphogenetic protein-2(BMP-2) and osteoprotegerin(OPG) improved osteogenesis and new bone formation by trans-sutural distraction osteogenesis. Methods Twenty four dogs were divided into three groups randomly and received new internal trans-sutural distraction osteogenesis treatment. Five days after operation, infusion apparatus with double-tube was inserted to submucosa near the distracted zone
to deliver controlled release agent of recombinant human bone morphogenetic protein-2/poly(lactic-co-glycolic acid)/fibrin sealant(rhBMP-2/PLGA/FS) in group A and group C. Recombinant human osteoprotegerin/fibrin sealant(rhOPG/FS) was injected three weeks later in group B and group C. Histology staining and bone histomorphometry were used to measure the changes of maxillary bone sutura after distraction for 1, 2, 4 and 6 weeks. Results New bone formation
observed in distracted zone showed a significant increase in group A and C. Transmission electron microscope showed the osteoblast and osteocyte were active with dilated rough endoplasmic reticulum and a large number of chondriosomes and Golgi complex. After distraction for 6 weeks, indexes of osteoblast of group A, B, and C were 38.5±7.7, 35.7±6.5, and 41.7±11.0, indexes of osteoclast(Ioc) were 5.9±1.0, 1.2±0.3, and 2.8±0.4, bone trabecula thicknesses were (38.36±13.28),(66.20±9.16), and(51.85±9.92)μm respectively. Increased bone density and decreased Ioc were found in group B and C. Conclusion The new elastic distractor is effective in inducing new bone formation. BMP-2 and OPG combination acts synergistically, and leads to significant enhancement of bone formation and remodeling.

Key words: distraction osteogenesis, bone morphogenetic protein-2, osteoprotegerin