Abstract:

Objective　To elucidate the molecular structure of pyruvate oxidase gene promoter.Methods　The 1·30 kb fragment with promoter activity, amplified from upstream ofStreptococcus oralispyruvate oxidase gene (Sopox), was cloned into vector PBK-CMV. The positive transformedE.coliJM109 clone was selected, the recombinant plasmidwas further identifiedwith restriction mapping analysis. The positive recombinant plasmid was studied with sequence analysis.Results　After digesting the recombinant plasmid withHindⅢ, 1% agarose electrophoresis showed 1·30 kb fragment, which was consistent with predicted size. Sequence analysis revealed 1 350 bp.Conclusion　TheSopoxpromoter region is sequenced. Further characterization of the Sopoxpromoter region will elucidate the molecular mechanism of H2O2production ofstreptococcus oralis.

Key words: Streptococcus oralis, pyruvate oxidase, pyruvate oxidase gene, promoter