Distribution of bacteria infected by metagenomic sequencing technology in maxillofacial space

doi:10.7518/hxkq.2021.04.016

Abstract

Abstract: Objective

This study aimed to compare and analyze the consistency and difference between metageno-mic next-generation sequencing (mNGS) and conventional bacterial culture in the detection of pathogenic microorganisms in maxillofacial space infection, as well as to provide a new detection method for the early clinical identification of pathogenic bacteria in maxillofacial space infection.

Methods

The clinical data of 16 patients with oral and maxillofacial space infections in the First Affiliated Hospital of Zhengzhou University from March 2020 to June 2020 were collected. mNGS and conventional bacterial culture methods were used to detect pus. We then analyzed and compared the test results of the two methods, including the test cycle, positive detection rate, anaerobic bacteria, facultative anaerobes and aerobic bacteria detection rates, distribution of pathogenic bacteria, relative species abundance, and resistance genes.

Results

The average inspection period of mNGS was (18.81±3.73) h, and the average inspection period of bacterial culture was (83.25±11.64) h, the former was shorter than the latter (P<0.05). The positive detection rate of mNGS was 100% (16/16), and the positive detection rate of conventional bacterial culture was 31.25% (5/16), the former was higher than the latter (P<0.05). The detection rate of mNGS anaerobic bacteria was 93.75% (15/16), the detection rate of bacterial culture anaerobes was 0 (0/16), the former was higher than the latter (P<0.05). Using mNGS, the detection rate of facultative anaerobes in bacterial culture was 75.00% (12/16), and the detection rate of facultative anaerobes in bacterial culture was 25.00% (4/16), the former was higher than the latter (P<0.05). The detection rate of aerobic bacteria in bacterial culture was 12.50% (1/16), the former was higher than the latter (P>0.05). mNGS detected 15 kinds of pathogenic bacteria, among which 3 were Gram positive, 12 were Gram negative, 49 were non-pathogenic, 16 were Gram positive, and 32 were Gram negative, 1 was fungus.

Conclusion

Compared with conventional bacterial culture, mNGS has the characteristics of short test time, high sensitivity, and high accuracy. Thus, it is a new detection method for the early identification of pathogenic bacteria in maxillofacial space infection and is beneficial to the early clinical diagnosis and treatment of the disease.

Key words: metagenomic next-generation sequencing, maxillofacial space infection, pathogenic microorga-nism, next-generation sequencing technology

CLC Number:

R 782.3

Chen Yiheng, Zheng Hongyu, Li Zixuan, Wu Yongchao, Niu Zhixing, Peng Yanhui, Zhao Junfang, Sun Qiang. Distribution of bacteria infected by metagenomic sequencing technology in maxillofacial space[J]. West China Journal of Stomatology, 2021, 39(4): 475-481.

Figures/Tables 3

References 30

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耐药药物种类	耐药基因
大环内酯类	ErmF、ErmB、lsaC、Erm（35）、H-NS
林可酰胺类	ErmF、ErmB、lsaC、Erm（35）
四环素类	tetM、tetS、lsaC、tetQ、emrY、AcrS、acrB、LAP- 2、H-NS
头霉素类	CfxA、CfxA2、CfxA3、CfxA5、H-NS
链霉素类	marA
氟喹诺酮类	H-NS、mdtH、emrA、emrR、acrB、LAP-2、QnrS1
头孢菌素类	H-NS、marA、AcrS、acrB
碳青霉烯类	lsaC、marA

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