Effects of large tumor suppressor homolog 2 gene overexpression on the proliferation and apoptosis of oral squamous cell carcinoma

doi:10.7518/hxkq.2018.06.005

Abstract

Abstract:

Objective To investigate the effect of large tumor suppressor homolog 2 (LATS2) gene overexpression on the proliferation and apoptosis of oral squamous cell carcinoma (OSCC).Methods Lentivirous particles were transferred into SCC-25 cell to upregulate LATS2 gene expression. Cell proliferation was detected by CCK-8 assay. Apoptosis was detected through flow cytometry. The expression changes of Bax, Bcl-2, and LATS2 were analyzed by Western blot.Results Gene transfection increased LATS2 expression. Compared with the control group and pEGFP-control group, SCC-25 cell proliferation in the pGFP-LATS2 group was inhibited, whereas the apoptosis ratio increased (P<0.05). Bcl-2 expression decreased, and Bax expression increased.Conclusion Overexpression of LATS2 could inhibit SCC-25 cell proliferation and induce apoptosis.

Key words: oral squamous cell carcinoma, large tumor suppressor homolog 2, proliferation, apoptosis

CLC Number:

Q786

Zengwen Yue,Shubin Wang,Jinzhong Liu. Effects of large tumor suppressor homolog 2 gene overexpression on the proliferation and apoptosis of oral squamous cell carcinoma[J]. West China Journal of Stomatology, 2018, 36(6): 609-612.

Figures/Tables 4

References 10

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