Preparation of multifunctional nanoparticles targeting tongue cancer and in vitro study

doi:10.7518/hxkq.2018.03.002

Abstract

Abstract:

Objective This study aims to prepare docetaxel (DOC)-loaded multifunctional nanoparticles containing indo-cyanine green (ICG) and perfluorohexane (PFH) as targeted drug delivery system, which is supplemented with stromal cell-derived factor-1 (SDF-1), and characterize their properties. Methods Multifunctional nanoparticles were prepared by using the double emulsion method. SDF-1 was covalently conjugated to the surface of the nanoparticles through thioe-ther bonding. Their particle size, distribution, and surface potential were determined with the Malvern measuring in-strument. The conjugation of SDF-1 was evaluated by confocal laser scanning microscope. Encapsulation efficiency (ELC), drug loading capacity (DLC), and release regularity of the nanoparticles were determined by high-performance liquid chro-matography (HPLC). In vitro photothermal property was recorded by a thermal imager. The in vitro imaging capacity was observed by a photoacoustic instrument and an ultrasonic diagnostic apparatus. Targeting capability was assessed by flow cytometry. The cell activity on SCC-15 cells was checked by CCK-8 method. Results The targeted multifunctional nano-particles showed regularly sphericity. The diameter was (502.88±17.92) nm. The zeta potential was (-11.5±3.15) mV. ELC was 54.12%±1.74%. DLC was 1.08 mg·mL^-1. In vitro drug release was initially fast and subsequently slow. The photothermal characteristics were related to the concentration; the higher the concentration, the higher the temperature. Nanoparticles could detect significant photoacoustic and ultrasound signals. The in vitro targeting rate was 89.99%. No significant differences of cell viability in the SINPs groups were observed at each concentration (P>0.05). The inhibition effect of DOC-SINPs was stronger than that of SINPs whether or not in the presence of laser irradiation among the groups of 150 and 200 μg·mL^-1 (P<0.05). Conclusion Multifunctional nanoparticles for diagnosis and treatment were successfully prepared and displayed dual-mode ultrasound/photoacoustic imaging and antitumor effects of chemotherapy and photothermal therapy.

Key words: poly (lactic-co-glycolic acid), indocyanine green, targeted therapy, tongue carcinoma, molecular imaging

CLC Number:

R739.8

Wei Ren, Lihua Qiu, Zhi Gao, Pan Li, Xin Zhao, Chengchen Hu. Preparation of multifunctional nanoparticles targeting tongue cancer and in vitro study[J]. West China Journal of Stomatology, 2018, 36(3): 240-246.

Figures/Tables 7

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