West China Journal of Stomatology

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The effect of basic fibroblast growth factor on the gene expr ession of decor in by per iodontal ligament fibroblasts in cultur e

WANG Si- cong1, LIN Chong- tao1, NIE Dai- bang2, OUYANG Hong- sheng2   

  1. 1. Dept. of Periodontology, Stomatology Hospital of Jilin University, Changchun 130041, China; 2. Dept. of Animal Biotechnology, School of Animal Science and Veterinary Medicine, Jilin University, Changchun 130062, China
  • Received:2008-08-25 Revised:2008-08-25 Online:2008-08-20 Published:2008-08-20
  • Contact: LIN Chong- tao,Tel:0431- 88796039

Abstract:

目的研究碱性成纤维细胞生长因子(bFGF)对人牙周膜成纤维细胞内核心蛋白多糖(decorin)的影响,探讨bFGF在牙周再生中的意义。方法体外原代培养人牙周膜成纤维细胞,用外源性bFGF刺激细胞,半定量RTPCR法检测牙周膜成纤维细胞内decorin基因表达的变化。结果电泳结果表明,bFGF抑制人牙周膜成纤维细胞内decorin的mRNA合成,并且随着质量浓度的增加抑制作用减弱。结论decorin具有很多生物学功能,bFGF对decorin的抑制作用很可能是牙周炎损伤修复过程中一个重要的调节因素,为bFGF在牙周组织再生中的作用提供理论基础。

Key words: basic fibroblast growth factor, decorin, periodontal ligament fibroblasts, periodontal regeneration