West China Journal of Stomatology

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Effect of or al car cinoma- associated fibroblasts on extr acellular signal- r egulated kinases pathway in the lingual car cinoma cell line

LIN Jing- wen1, CHEN Qian- ming2, LI Sheng- fu3, FU Chun- hua1, LIN Mei2, LONG Dan3, ZHOU Hong- mei2   

  1. 1. Key. Laboratory of Oral Biomedical Engineering of Ministry of Education, Sichuan University, Chengdu 610041, China；2. Dept. of Oral Medicine, West China College of Stomatology, Sichuan University, Chengdu 610041, China; 3. Key. Laboratory of Transplantation Engineering and Transplantation Immunity of Ministry of Health, West China Hospital, Sichuan University, Chengdu 610041, China
  • Received:2007-10-25 Revised:2007-10-25 Online:2007-10-20 Published:2007-10-20
  • Contact: ZHOU Hong- mei，Tel：028- 85503480

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Abstract:

Objective To investigate the effects of oral carcinoma - associated fibroblasts（CAFs） on extracellular signal- regulated kinases（ERK） pathway in a lingual carcinoma cell line. Methods The lingual carcinoma cell line. Tca8113 was stimulated by conditioned medium from oral CAFs, or cocultured with oral CAFs for definite time. Total ERK and pERK in Tca8113 lysate were detected by Western blotting, and the ratio between pERK and ERK were calculated. Results Both stimulation by conditioned medium and coculture induced prompt phosphorylation of ERK, and increased the ratio between pERK and ERK. Conclusion Oral CAFs can activate ERK pathway of carcinoma cells.

Key words: carcinoma- associated fibroblasts, tongue carcinoma, extracellular signal- regulated kinases pathway

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