West China Journal of Stomatology

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Cloning of human bone morphogenetic protein - 2 gene and the construction of its eukaryotic expr ession vector

ZHOU Nuo, HUANG Xuan- ping, LIAO Ni, WEI Shan- liang, LIANG Fei - xin, MAI Hua- ming   

  1. Dept. of Oral and Maxillofacial Surgery, Hospital of Stomatology, Guangxi Medical University, Nanning 530021, China
  • Received:2007-10-25 Revised:2007-10-25 Online:2007-10-20 Published:2007-10-20
  • Contact: ZHOU Nuo,Tel:0771- 5358645

Abstract:

目的克隆人骨形态发生蛋白- 2(hBMP2)基因片段,构建pcDNA3.1- hBMP2真核表达质粒。方法采用逆转录聚合酶链式反应(RT- PCR)技术,从人骨肉瘤中扩增出人骨形态发生蛋白- 2基因片段,通过DNA重组技术将该基因片段重组于pcDNA3.1真核表达载体上,构建pcDNA3.1- hBMP2重组质粒,通过用PCR扩增、酶切电泳分析及DNA测序的方法对重组DNA进行鉴定。结果经PCR扩增、酶切电泳分析和DNA测序证实,本实验构建的重组质粒目的基因片段为人BMP2- cDNA。结论本实验成功克隆了hBMP2基因并构建成其真核表达质粒。

Key words: 骨形态发生蛋白- 2, 基因克隆, 真核表达载体