West China Journal of Stomatology

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Effects of fluid shear str ess str ength on mRNA expr ession of ATP6V1a1 in polar ized osteoclasts

ZHANG Qing- hong1，2, LIANG Xing2, LIU Meng- tao3, ZHU Bao- min2, FU Jun2   

  1. 1. Dept. of Prosthodontics, The Affiliated Stomatology Hospital of Medical College, Zhejiang University, Hangzhou 310006, China; 2. Key. Laboratory of Oral Biomedical Engineering of Ministry of Education, Sichuan University, Chengdu 610041, China；3. Dept. of Prosthodontics, The Red Cross Hospital of Kunming, Kunming 650021, China
  • Received:2007-08-25 Revised:2007-08-25 Online:2007-08-20 Published:2007-08-20
  • Contact: LIANG Xing, Tel：028- 85503570

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Abstract:

Objective To observe effects of fluid shear stress strength on mRNA expression of ATP6V1a1 in rat polarized osteoclasts. Methods Rat polarized osteoclasts suffered 0.0（control group）, 0.9, 2.9, 8.7 and 26.3 dynes/cm2 fluid shear stress for 30 min. mRNA expression of ATP6V1a1 was detected by Real - Time fluorescent quantitation PCR. Results mRNA expression of ATP6V1a1 in 0.0（control group）, 0.9，2.9，8.7 and 26.3 dynes/cm2 groups was （1.14±0.06）×106, （1.62±0.09）×106, （2.28±0.13）×106, （3.24±0.18）×106, （9.16±0.53）×106 copy numbers, respectively （P<0.05）. Conclusion In the present study, polarized osteoclasts are sensitive to fluid shear stress. mRNA expression of ATP6V1a1 has increscent tendency along with increasing of fluid shear stress strength.

Key words: osteoclast, fluid shear stress strength, bone resorption

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