Effects of Porphyromonas gingivalis lipopolysacchar ide on collagen phagocytosis by human per iodontal ligament fibroblasts

West China Journal of Stomatology

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Effects of Porphyromonas gingivalis lipopolysacchar ide on collagen phagocytosis by human per iodontal ligament fibroblasts

FANG Chang- yun1, SU Zheng1, CHEN Lei1, FAN Ming- wen2, JIAN Xin- chun1, CHEN Zhi2

1. Stomatological Department, Xiangya Hospital of Central South University, Changsha 410008, China; 2. Dept. of Operative Dentistry, Cariology and Endodontology, Stomatological College of Wuhan University, Wuhan 430079, China

Received:2007-08-25 Revised:2007-08-25 Online:2007-08-20 Published:2007-08-20
Contact: FANG Chang- yun，Tel：13787319797

Abstract

Abstract:

Objective To investigate the effects of Porphyromonas gingivalis（P.gingivalis） lipopolysaccharide（LPS） on collagen phagocytosis of human periodontal ligament fibroblasts（hPDLF）. Methods Fluorescence localization and flow cytometry was used to test the collagen phygocytosis of hPDLF stimulated by the various concentration of LPS for 48 hours in vitro. Results The collagen phygocytosis of hPDLF was increased significantly by 0.1 μg/mL LPS （P <0.05）. Conclusion This study indicates that P.gingivalis LPS may enhance the degradation of collagen by stimulating the phagocytic activity of the hPDLF in periodontitis.

Key words: Porphyromonas gingivalis, lipopolysaccharide, periodontal ligament fibroblasts, collagen, phagocytosis

FANG Chang- yun1, SU Zheng1, CHEN Lei1, FAN Ming- wen2, JIAN Xin- chun1, CHEN Zhi2. Effects of Porphyromonas gingivalis lipopolysacchar ide on collagen phagocytosis by human per iodontal ligament fibroblasts[J]. West China Journal of Stomatology.

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Effects of Porphyromonas gingivalis lipopolysacchar ide on collagen phagocytosis by human per iodontal ligament fibroblasts

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